Hleb Ekaterina Y, Hafner Jason H, Myers Jeffrey N, Hanna Ehab Y, Rostro Betty C, Zhdanok Sergey A, Lapotko Dmitri O
A.V. Lykov Heat & Mass Transfer Institute, 15 P. Brovka Street, Minsk, 220072, Belarus.
Nanomedicine (Lond). 2008 Oct;3(5):647-67. doi: 10.2217/17435889.3.5.647.
We have developed a method, termed laser-activated nano-thermolysis as a cell elimination technology (LANTCET), for the selective detection and destruction of individual tumor cells by the generation of intracellular photothermal bubbles around clusters of gold nanoparticles.
Bare nanoparticles and their conjugates to C225 tumor-specific monoclonal antibodies were applied in vitro to C225-positive squamous carcinoma cells and in vivo to an experimental tumor in a rat in order to form intracellular clusters of nanoparticles.
Single 10 ns laser pulses generated intracellular photothermal microbubbles at a near-infrared and visible wavelengths. The cells with the clusters yielded an almost 100-fold decrease in the laser fluence threshold for bubble generation and cell damage relative to that for the cells without clusters. Cell damage had a mechanical origin and single cell selectivity. Three LANTCET processes (cell detection, damage and optical guidance) were realized as a microsecond sequence and with the one device.
我们开发了一种方法，称为激光激活纳米热解作为细胞消除技术（LANTCET），通过在金纳米颗粒簇周围产生细胞内光热气泡来选择性检测和破坏单个肿瘤细胞。
将裸纳米颗粒及其与C225肿瘤特异性单克隆抗体的缀合物体外应用于C225阳性鳞状癌细胞，并体内应用于大鼠的实验性肿瘤，以形成纳米颗粒的细胞内簇。
单个10纳秒激光脉冲在近红外和可见光波长下产生细胞内光热微泡。与没有簇的细胞相比，有簇的细胞产生气泡和细胞损伤的激光能量密度阈值降低了近100倍。细胞损伤起源于机械作用且具有单细胞选择性。三个LANTCET过程（细胞检测、损伤和光学引导）以微秒级顺序通过一个装置实现。
