Rho激酶抑制剂Y27632和H1152在有培养的雪旺细胞存在的情况下可增强神经突延伸。

Rho kinase inhibitors Y27632 and H1152 augment neurite extension in the presence of cultured Schwann cells.

作者信息

Fuentes Erick O, Leemhuis Jost, Stark G Björn, Lang Eva M

机构信息

Department of Plastic and Hand Surgery, University of Freiburg Medical Centre, Freiburg, Germany.

出版信息

J Brachial Plex Peripher Nerve Inj. 2008 Sep 25;3:19. doi: 10.1186/1749-7221-3-19.

BACKGROUND

RhoA and Rho kinase inhibitors overcome the inhibition of axonal regeneration posed by central nervous system (CNS) substrates.

METHODS

To investigate if inhibition of the Rho pathway augments the neurite extension that naturally occurs in the peripheral nervous system (PNS) following nerve damage, dorsal root ganglion neurons and Schwann cell co-cultures were incubated with culture medium, C3 fusion toxin, and the Rho kinase (ROCK) inhibitors Y27632 and H1152. The longest neurite per neuron were measured and compared. Incubation with Y27632 and H1152 resulted in significantly longer neurites than controls when the neurons were in contact with Schwann cells. When separated by a porous P.E.T. membrane, only the group incubated with H1152 developed significantly longer neurites. This work demonstrates that Rho kinase inhibition augments neurite elongation in the presence of contact with a PNS-like substrate.

背景

RhoA和Rho激酶抑制剂可克服中枢神经系统（CNS）底物对轴突再生的抑制作用。

方法

为研究抑制Rho信号通路是否会增强神经损伤后周围神经系统（PNS）中自然发生的神经突延伸，将背根神经节神经元和雪旺细胞共培养物与培养基、C3融合毒素以及Rho激酶（ROCK）抑制剂Y27632和H1152一起孵育。测量并比较每个神经元最长的神经突。当神经元与雪旺细胞接触时，用Y27632和H1152孵育导致神经突明显长于对照组。当用多孔聚对苯二甲酸乙二酯（P.E.T.）膜隔开时，只有用H1152孵育的组神经突明显更长。这项研究表明，在与类似PNS的底物接触的情况下，抑制Rho激酶可增强神经突伸长。