Roguev Assen, Bandyopadhyay Sourav, Zofall Martin, Zhang Ke, Fischer Tamas, Collins Sean R, Qu Hongjing, Shales Michael, Park Han-Oh, Hayles Jacqueline, Hoe Kwang-Lae, Kim Dong-Uk, Ideker Trey, Grewal Shiv I, Weissman Jonathan S, Krogan Nevan J
Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA.
Science. 2008 Oct 17;322(5900):405-10. doi: 10.1126/science.1162609. Epub 2008 Sep 25.
An epistasis map (E-MAP) was constructed in the fission yeast, Schizosaccharomyces pombe, by systematically measuring the phenotypes associated with pairs of mutations. This high-density, quantitative genetic interaction map focused on various aspects of chromosome function, including transcription regulation and DNA repair/replication. The E-MAP uncovered a previously unidentified component of the RNA interference (RNAi) machinery (rsh1) and linked the RNAi pathway to several other biological processes. Comparison of the S. pombe E-MAP to an analogous genetic map from the budding yeast revealed that, whereas negative interactions were conserved between genes involved in similar biological processes, positive interactions and overall genetic profiles between pairs of genes coding for physically associated proteins were even more conserved. Hence, conservation occurs at the level of the functional module (protein complex), but the genetic cross talk between modules can differ substantially.
通过系统测量与成对突变相关的表型，在裂殖酵母粟酒裂殖酵母中构建了一个上位性图谱（E-MAP）。这个高密度的定量遗传相互作用图谱聚焦于染色体功能的各个方面，包括转录调控和DNA修复/复制。E-MAP发现了RNA干扰（RNAi）机制中一个以前未被识别的组分（rsh1），并将RNAi途径与其他几个生物学过程联系起来。将粟酒裂殖酵母的E-MAP与来自芽殖酵母的类似遗传图谱进行比较，结果显示，虽然参与相似生物学过程的基因之间的负相互作用是保守的，但编码物理相关蛋白质的基因对之间的正相互作用和整体遗传图谱更加保守。因此，保守性发生在功能模块（蛋白质复合体）水平，但模块之间的遗传相互作用可能有很大差异。
