Hill Katherine L, Kaufman Dan S
Stem Cell Institute and Department of Medicine, University of Minnesota, Minneapolis, Minnesota, USA.
Curr Protoc Stem Cell Biol. 2008 Sep;Chapter 1:Unit 1F.6. doi: 10.1002/9780470151808.sc01f06s6.
Human embryonic stem (hES) cells are of remarkable interest both for the utility of these cells for studying basic human developmental biology and as a potential source for novel therapeutics. Here, we provide detailed methodologies of one of the first systems used to mediate differentiation of hES cells--stromal cell coculture. Use of stromal cells adds the ability to manipulate aspects of the developmental niche that support differentiation into a defined lineage. These methods will allow efficient and reproducible development of hematopoietic progenitor cells, as well as potentially mature hematopoietic cells that are suitable for subsequent in vitro and in vivo studies.
人类胚胎干细胞（hES细胞）因其在研究人类基础发育生物学方面的效用以及作为新型疗法的潜在来源而备受关注。在此，我们提供了最早用于介导hES细胞分化的系统之一——基质细胞共培养的详细方法。使用基质细胞增加了操纵支持分化为特定谱系的发育微环境各方面的能力。这些方法将有助于高效且可重复地培养造血祖细胞，以及潜在地培养出适合后续体外和体内研究的成熟造血细胞。
