Kage R, O'Harte F, Thim L, Conlon J M
Clinical Research Group for Gastrointestinal Endocrinology of the Max-Planck-Gesellschaft, University of Gottingen, F.R.G.
Regul Pept. 1991 Apr 25;33(2):191-8. doi: 10.1016/0167-0115(91)90213-z.
The rabbit small intestine contains neuromedin U-like immunoreactivity (22 pmol/g wet tissue weight) that was resolved into a single major molecular form by reversed-phase HPLC. The primary structure of the peptide was established as: Phe-Pro-Val-Asp-Glu-Glu-Phe-Gln-Ser-Pro10-Phe-Gly-Ser-Arg-Ser-Arg- Gly-Tyr-Phe- Leu20-Phe-Arg-Pro-Arg-Asn.NH2. In rabbit neuromedin U, the Arg16-Arg17 dibasic residue processing site that is found in pig and dog neuromedin U-25 is replaced by Arg-Gly, but this potential monobasic processing site is not utilized by cleavage enzyme(s) in the intestine.
兔小肠含有神经介素U样免疫反应性物质（22皮摩尔/克湿组织重量），通过反相高效液相色谱法可将其分离为单一主要分子形式。该肽的一级结构确定为：苯丙氨酸-脯氨酸-缬氨酸-天冬氨酸-谷氨酸-谷氨酸-苯丙氨酸-谷氨酰胺-丝氨酸-脯氨酸10-苯丙氨酸-甘氨酸-丝氨酸-精氨酸-丝氨酸-精氨酸-甘氨酸-酪氨酸-苯丙氨酸-亮氨酸20-苯丙氨酸-精氨酸-脯氨酸-精氨酸-天冬酰胺。NH2。在兔神经介素U中，猪和狗神经介素U-25中存在的精氨酸16-精氨酸17二碱性残基加工位点被精氨酸-甘氨酸取代，但这种潜在的单碱性加工位点未被肠道中的裂解酶利用。
