An Byungrak, Lee Gyusang, Lim Kwanhun, Moon Byung Soo, Kim Jong Bae
Department of Biomedical Laboratory Science, College of Health Science, Yonsei University, Wonju, Korea.
Microbiol Immunol. 2008 Oct;52(10):479-83. doi: 10.1111/j.1348-0421.2008.00064.x.
Various tyrosine phosphorylation motif regions of H. pylori cagA exist. The number of these regions was found to have some influence on cell signaling, which was found to be more pronounced when in D (ESS) region than in C (WSS) region. A molecular biological method with multiplex PCR was developed to distinguish C and D regions, and to identify the repetition number of tyrosine phosphorylation of the cagA gene. Multiplex PCR using novel primer sets was performed on 73 strains of H. pylori isolated from Korean patients with upper gastrointestinal diseases. The Western cagA was identified in only 3 strains (4.1%) whereas East Asia cagA was identified in 69 strains (94.5%). These results were reconfirmed through a sequencing analysis. The method developed in this study would be useful for monitoring the repeated number of C and D regions of tyrosine phosphorylation motifs in H. pylori cagA.
幽门螺杆菌cagA存在多种酪氨酸磷酸化基序区域。发现这些区域的数量对细胞信号传导有一定影响，且在D（ESS）区域比在C（WSS）区域时更为明显。开发了一种多重PCR的分子生物学方法来区分C和D区域，并鉴定cagA基因酪氨酸磷酸化的重复次数。使用新型引物对从韩国上消化道疾病患者中分离出的73株幽门螺杆菌进行多重PCR。仅在3株菌株（4.1%）中鉴定出Western cagA，而在69株菌株（94.5%）中鉴定出东亚cagA。这些结果通过测序分析得到再次证实。本研究中开发的方法将有助于监测幽门螺杆菌cagA中酪氨酸磷酸化基序的C和D区域的重复次数。
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