Qingdao Nucleic Acid Rapid Testing International Science and Technology Cooperation Base, College of Life Sciences, Department of Pathogenic Biology, School of Basic Medicine, and Department of Clinical Laboratory, The Affiliated Hospital of Qingdao University, Qingdao University, Qingdao, 266071, Shandong, China.
Key Laboratory of Optic-Electric Sensing and Analytical Chemistry for Life Science, MOE,, Shandong Provincial Key Laboratory of Biochemical Engineering, Qingdao Nucleic Acid Rapid Detection Engineering Research Center, College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao, 266042, People's Republic of China.
Curr Microbiol. 2022 Jun 29;79(8):235. doi: 10.1007/s00284-022-02931-4.
Helicobacter pylori cagA + genotype is a leading risk factor for gastric cancer development making accurate identification and timely eradication of H. pylori critical to deadly gastric cancer prevention. Traditional clinical diagnostic methods, including conventional in vitro culture, histological examination, and (13/14)C-urea breath test methods, could only identify the presence of H. pylori, but these means are not capable of identification of cagA + strains. Herein, we firstly built a multiplex detection system based on novel accelerated PCR that could realize one-step detection of as low as 20 copies of H. pylori 16S rDNA and cagA genes within 30 min. In addition, this novel system performed strong anti-jamming capacity, and exhibited that it could specifically differentiate H. pylori cagA- and cagA + genotypes co-existence with other 4 kinds of gastrointestinal pathogens. Furthermore, this one-step system showed remarkable performance on rapid H. pylori infection diagnosis and cagA + genotypes identification in clinical gastric mucosa samples. Specifically, it outperformed histological examination in terms of accuracy and was superior to conventional PCR and DNA sequencing in terms of efficiency. This rapid, sensitive, and reliable H. pylori detection and identification system would break the limitation of traditional methods and realize H. pylori infection diagnosis and cagA + genotypes identification.
幽门螺杆菌 cagA + 基因型是胃癌发展的主要危险因素，因此准确识别和及时根除幽门螺杆菌对于预防致命性胃癌至关重要。传统的临床诊断方法，包括常规体外培养、组织学检查和 (13/14)C-尿素呼气试验方法，只能识别幽门螺杆菌的存在，但这些方法无法识别 cagA + 菌株。在此，我们首次建立了一种基于新型加速 PCR 的多重检测系统，能够在 30 分钟内一步检测低至 20 个拷贝的幽门螺杆菌 16S rDNA 和 cagA 基因。此外，该新型系统具有强大的抗干扰能力，能够特异性地区分同时存在的幽门螺杆菌 cagA-和 cagA + 基因型与其他 4 种胃肠道病原体。此外，该一步法系统在临床胃黏膜样本中对快速幽门螺杆菌感染诊断和 cagA + 基因型鉴定具有出色的性能。具体来说，该系统在准确性方面优于组织学检查，在效率方面优于传统 PCR 和 DNA 测序。这种快速、敏感和可靠的幽门螺杆菌检测和鉴定系统将打破传统方法的局限性，实现幽门螺杆菌感染诊断和 cagA + 基因型鉴定。
