孟加拉国无16S rRNA基因突变的四环素耐药临床幽门螺杆菌的分离
Isolation of tetracycline-resistant clinical Helicobacter pylori without mutations in 16S rRNA gene in Bangladesh.
作者信息
Khan Rasel, Nahar Shamsun, Mukhopadhyay Asish K, Berg Douglas E, Ahmad Mian Mashhud, Okamoto Keinosuke, Nair G Balakrish, Rahman Motiur
机构信息
Laboratory Sciences Division, ICDDR,B: Centre for Health and Population Research, Dhaka, Bangladesh.
出版信息
Microbiol Immunol. 2008 Oct;52(10):508-11. doi: 10.1111/j.1348-0421.2008.00062.x.
The occurrence of 16S rRNA gene mutations associated with resistance to tetracycline in H. pylori isolated in Bangladesh was investigated. Tetracycline susceptibility was determined by the agar dilution method. The 16S rRNA genes of these isolates were sequenced and analyzed. A tetracycline accumulation assay was performed. DNA sequence and transformation tests of nine tetracycline-resistant (MIC = 2 microg/ml) Bangladeshi H. pylori clinical isolates showed that in no case was the resistance due to mutations in the 16S rRNA gene, the only known cause of tetracycline resistance in this pathogen. Tetracycline accumulation assays implicated altered uptake or efflux.
对在孟加拉国分离出的幽门螺杆菌中与四环素耐药性相关的16S rRNA基因突变的发生情况进行了调查。采用琼脂稀释法测定四环素敏感性。对这些分离株的16S rRNA基因进行测序和分析。进行了四环素积累试验。对9株四环素耐药（MIC = 2微克/毫升）的孟加拉国幽门螺杆菌临床分离株进行的DNA序列和转化试验表明，在任何情况下，耐药性都不是由16S rRNA基因突变引起的，而16S rRNA基因突变是该病原体中已知的唯一四环素耐药原因。四环素积累试验表明摄取或外排发生了改变。
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