[力达霉素诱导人胃癌BGC823细胞凋亡并抑制裸鼠移植瘤生长]
[Lidamycin induces apoptosis of human gastric carcinoma BGC823 cells and inhibits xenograft growth in nude mice].
作者信息
Zhang Sheng-Hua, Chen Jing, Jiang Ming, Zhen Yong-Su
机构信息
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
出版信息
Yao Xue Xue Bao. 2008 Jun;43(6):601-4.
To investigate the effect of lidamycin (LDM) on human gastric carcinoma BGC823 cells and xenograft growth in nude mice, MTT (methyl thiazolyl tetrazolium) assay was used to determine the inhibition of BGC823 cell proliferation by LDM. Induction of apoptosis was studied by flow cytometry and TUNEL assay. The expression of VEGF was detected by Western blotting analysis. Athymic nude mice were used to determine in vivo antitumor activity. Proliferation inhibition and apoptosis induction were studied in lidamycin-treated cells. The expression of VEGF in BGC823 cells decreased in a dose-dependent manner. LDM at 0.02 mg x kg(-1) and 0.04 mg x kg(-1) suppressed the growth of BGC823 xenografts in nude mice by 57% and 72%, respectively. LDM potently induces apoptosis in human gastric carcinoma BGC823 cells and inhibits xenograft growth.
为研究力达霉素（LDM）对人胃癌BGC823细胞及裸鼠移植瘤生长的影响，采用MTT（噻唑蓝）法测定LDM对BGC823细胞增殖的抑制作用。通过流式细胞术和TUNEL法研究细胞凋亡的诱导情况。采用蛋白质免疫印迹分析检测血管内皮生长因子（VEGF）的表达。利用无胸腺裸鼠测定体内抗肿瘤活性。研究力达霉素处理的细胞中的增殖抑制和凋亡诱导情况。BGC823细胞中VEGF的表达呈剂量依赖性降低。0.02 mg·kg⁻¹和0.04 mg·kg⁻¹的LDM分别使裸鼠体内BGC823移植瘤的生长抑制了57%和72%。LDM可有效诱导人胃癌BGC823细胞凋亡并抑制移植瘤生长。
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