Mouchel-Vielh Emmanuèle, Bloyer Sébastien, Salvaing Juliette, Randsholt Neel B, Peronnet Frédérique
UMR 7622-Biologie du Développement; CNRS-Université Pierre et Marie Curie Paris 6 (UPMC), 9, quai Saint-Bernard, 75005 Paris, France.
Genes Cells. 2008 Nov;13(11):1099-111. doi: 10.1111/j.1365-2443.2008.01231.x. Epub 2008 Sep 23.
Mitogen-activated protein kinase (MAPK) cascades are evolutionary conserved transduction pathways involved in many cellular processes. Kinase modules are associated with scaffold proteins that regulate signaling by providing critical spatial and temporal specificities. Some of these scaffold proteins have been shown to be conserved, both in sequence and function. In mouse, the scaffold MP1 (MEK Partner 1) forms a signaling complex with MEK1 and ERK1. In this work, we focus on Drosophila MP1 (dMP1). We show that dMP1 is expressed ubiquitously during embryonic and larval development. By in vitro and in vivo experiments, we show that dMP1 is located in the cytoplasm and the nuclei, and that it interacts with MEK and ERK. Genetic studies with transgenic Drosophila lines allowing either dMP1 over-expression or dMP1 down-regulation by RNA interference highlight dMP1 function in the control of cell differentiation during development of the Drosophila wing.
丝裂原活化蛋白激酶（MAPK）级联反应是参与许多细胞过程的进化保守转导途径。激酶模块与支架蛋白相关联，这些支架蛋白通过提供关键的空间和时间特异性来调节信号传导。其中一些支架蛋白在序列和功能上都已被证明是保守的。在小鼠中，支架蛋白MP1（MEK伴侣1）与MEK1和ERK1形成信号复合物。在这项工作中，我们聚焦于果蝇MP1（dMP1）。我们发现dMP1在胚胎和幼虫发育过程中普遍表达。通过体外和体内实验，我们发现dMP1定位于细胞质和细胞核中，并且它与MEK和ERK相互作用。利用转基因果蝇品系进行的遗传学研究，通过RNA干扰实现dMP1的过表达或下调，突出了dMP1在果蝇翅膀发育过程中对细胞分化控制的功能。
