Mukhopadhyay Ananda, De Damayanti
Department of Zoology, University of North Bengal, Darjeeling 734 013, India.
J Invertebr Pathol. 2009 Jan;100(1):57-60. doi: 10.1016/j.jip.2008.08.006. Epub 2008 Sep 13.
A granulosis virus (GV) was isolated from the diseased caterpillars of Arctornis submarginata (Walker) (Lymantriidae), a defoliating pest of tea from Darjeeling foothill region. The phase contrast and transmission electron microscopic studies identified the virus as granulosis virus. SDS-PAGE analysis of major protein of the occlusion bodies was found to be 31 kDa, characteristic for granulin. The total genomic DNA was isolated. The major band found was of molecular weight 16 kDa. Bioassay conducted with the occlusion bodies (OBs) of the virus showed LC(50) value of 4.46 x 10(4) OBs/ml for the second instar caterpillars. Median lethal time (LT(50)) were 6.6 days for 1 x 10(4)OBs/ml, 5.09 days for 1 x 10(5) OBs/ml, 4.45 days for 1 x 10(6) OBs/ml and 3.87 days for 1 x 10(7) OBs/ml concentrations. The results indicated the potential of the virus for its future application as microbial pesticide against A. submarginata in future.
从大吉岭山麓地区一种食叶性茶树害虫——亚缘弧夜蛾（Walker，灯蛾科）的患病幼虫中分离出一种颗粒体病毒（GV）。通过相差显微镜和透射电子显微镜研究确定该病毒为颗粒体病毒。对包涵体主要蛋白进行的SDS-PAGE分析发现其分子量为31 kDa，这是颗粒体蛋白的特征。分离出了病毒的总基因组DNA。发现的主要条带分子量为16 kDa。用该病毒的包涵体（OBs）进行的生物测定表明，对二龄幼虫的LC(50)值为4.46×10(4)个OBs/ml。对于1×10(4)个OBs/ml、1×10(5)个OBs/ml、1×10(6)个OBs/ml和1×10(7)个OBs/ml浓度，中位致死时间（LT(50)）分别为6.6天、5.09天、4.45天和3.87天。结果表明该病毒未来有作为微生物杀虫剂防治亚缘弧夜蛾的潜力。
