Khodyrev D S, Loginov V I, Pronina I V, Kazubskaia T P, Gar'kavtseva R F, Braga E A
Genetika. 2008 Aug;44(8):1126-32.
The protein encoded by RAR-beta (retinoic acid receptor) gene is a member of the superfamily, of nuclear receptors of retinoids which are involved in regulation of cell differentiation and proliferation. The level of RAR-beta2 mRNA is downregulated in a number of cell lines derived from human epithelial tumors. Inactivation of the RAR-beta2 gene is associated with methylation of its promoter region, which is observed in various carcinomas at a frequency of 30-70%. In renal and ovarian tumors, methylation at this region is poorly studied, the data being contradictory. We report a high methylation frequency in the gene promoter region in RCC (59%, 36/61) and a somewhat lower frequency in EOC (30%, 15/50). Methylation frequency in BC (46%, 26/56) is consistent with the published data. Significant correlation of methylation frequency in promoter region of RAR-beta2 gene with RCC progression (P < or = 0.005 by Fisher's exact test) was established.
维甲酸受体β（RAR-β）基因编码的蛋白质是类视黄醇核受体超家族的成员，参与细胞分化和增殖的调控。在许多源自人类上皮肿瘤的细胞系中，RAR-β2 mRNA水平下调。RAR-β2基因的失活与其启动子区域的甲基化有关，在各种癌症中，这种甲基化的发生率为30%-70%。在肾肿瘤和卵巢肿瘤中，该区域的甲基化研究较少，数据相互矛盾。我们报告肾细胞癌（RCC）中该基因启动子区域的甲基化频率较高（59%，36/61），而在卵巢上皮癌（EOC）中甲基化频率略低（30%，15/50）。乳腺癌（BC）中的甲基化频率（46%，26/56）与已发表的数据一致。通过Fisher精确检验确定，RAR-β2基因启动子区域的甲基化频率与肾细胞癌进展显著相关（P≤0.005）。
