[A型产气荚膜梭菌毒素及其经凝胶过滤和离子交换色谱法获得的组分的白细胞毒性活性研究]
[Study of leukotoxic activity of Cl. perfringens type A toxin and its fractions obtained by gel filtration and ion exchange chromatography].
作者信息
Tselukh A V, Protchenko P Z, Golovatiuk A L
出版信息
Zh Mikrobiol Epidemiol Immunobiol. 1976 Jul(7):119-23.
In gel-filtration of Cl. perfringens type A toxin on Sephadex F-100 and F-50 there was revealed relationship between the leukotoxic activity and a high-molecular component. A method of ion-exchange chromatography on a column with DEAE-Sephadex A-25 from the Cl. perfringens toxin there were obtained 8 fractions 3 of which possessed a marked leukotoxic activity: the capacity to destroy neutrophils in the Svejcar and Vancurik test and to-depress the phagocytic activity of leukocytes. Lecithinase and lethal activity was revealed in one of these fractions only. Leukotoxic fractions differed by the capacity to destroy neutrophils and to decrease their phagocytic activity.
在用葡聚糖凝胶F - 100和F - 50对A型产气荚膜梭菌毒素进行凝胶过滤时，发现白细胞毒性活性与一种高分子组分之间存在关联。采用装有二乙氨基乙基葡聚糖A - 25的离子交换柱对产气荚膜梭菌毒素进行色谱分析，得到了8个组分，其中3个组分具有显著的白细胞毒性活性：在斯韦伊卡尔和万库里克试验中具有破坏中性粒细胞的能力，并能抑制白细胞的吞噬活性。仅在其中一个组分中发现了卵磷脂酶和致死活性。白细胞毒性组分在破坏中性粒细胞的能力以及降低其吞噬活性方面存在差异。
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