Gnanamani A, Kavitha V, Sekaran G, Rajakumar G Suseela
Microbiology Division, Central Leather Research Institute (Council of Scientific and Industrial Research, New Delhi), Adyar, Chennai, Tamil Nadu, India. agmani
Colloids Surf B Biointerfaces. 2008 Dec 1;67(2):192-8. doi: 10.1016/j.colsurfb.2008.08.016. Epub 2008 Aug 26.
The present study demonstrates the role of microbial hydrolases in the transformation of hydrocarbons (soybean, sunflower, groundnut and gingelly oil, etc.) to vesicles. The combined effect of lipolytic enzyme generation and biosurfactants production during microbial growth at optimized media and environmental conditions mediates this transformation. Among the microbial species, Candida albicans exhibit complete transformation compared to Pseudomonads and Bacillus sps. Within hydrocarbons, only soybean and sunflower oils transformed to solid mass and no change with the remaining oils. Characterization of the vesicles revealed an increase in total weight by 160-180% compared to the original weight of hydrocarbon taken for the study and more than 73% increases in viscosity. Acid value and saponification value also showed an increase, respectively, by 78 and 84%. The bound water content estimated was 26%. Light microscopic analysis exhibit, presence of unilamellar and bi-lamellar structures.
本研究证明了微生物水解酶在将碳氢化合物（大豆油、向日葵油、花生油和芝麻油等）转化为囊泡过程中的作用。在优化的培养基和环境条件下微生物生长期间，脂肪分解酶生成和生物表面活性剂产生的联合作用介导了这种转化。在微生物物种中，与假单胞菌属和芽孢杆菌属相比，白色念珠菌表现出完全转化。在碳氢化合物中，只有大豆油和向日葵油转化为固体物质，其余油类没有变化。囊泡的表征显示，与用于该研究的碳氢化合物原始重量相比，总重量增加了160 - 180%，粘度增加了73%以上。酸值和皂化值也分别增加了78%和84%。估计的结合水含量为26%。光学显微镜分析显示存在单层和双层结构。
