DeBowes L J, Anderson N V
Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan 66506.
Am J Vet Res. 1991 Jun;52(6):869-72.
The effect of systemic administration of glucocorticoids was evaluated on 3 populations of macrophages obtained from healthy dogs. Phagocytic and Fc-receptor activities were determined for mononuclear phagocytes from blood and pulmonary and peritoneal lavage fluids. Samples were collected from 12 dogs before treatment and again on the same dogs after glucocorticoid administration. Thirty or more days were allowed between treatment periods. Twelve hours after combined prednisolone sodium succinate and dexamethasone sodium phosphate administration, the percentage of phagocytosing cells decreased for blood monocytes and increased for pulmonary macrophages. The percentage of pulmonary macrophages positive for erythrocyte antibody-rosette formation (Fc-receptor activity) increased. After 7 days of oral administration of prednisone, the percentage of phagocytosing peritoneal macrophages increased, whereas the percentage of blood monocytes with Fc-receptor activity increased. Results indicate that significant (P less than 0.05) changes in macrophage function occur after systemic administration of the glucocorticoid doses used in this study. Also, the effect of systemic administration of glucocorticoids on mononuclear phagocytes varies, depending on the specific cell location.
对从健康犬获取的3组巨噬细胞评估了全身给予糖皮质激素的效果。测定了来自血液以及肺和腹腔灌洗液的单核吞噬细胞的吞噬活性和Fc受体活性。在治疗前从12只犬采集样本，在给予糖皮质激素后对同一些犬再次采集样本。两个治疗期之间间隔30天或更长时间。在联合给予琥珀酸泼尼松龙和地塞米松磷酸钠12小时后，吞噬细胞的百分比对于血液单核细胞降低，而对于肺巨噬细胞升高。红细胞抗体玫瑰花结形成阳性（Fc受体活性）的肺巨噬细胞百分比增加。在口服泼尼松7天后，吞噬性腹腔巨噬细胞的百分比增加，而具有Fc受体活性的血液单核细胞百分比增加。结果表明，在本研究中使用的糖皮质激素剂量全身给药后，巨噬细胞功能发生了显著（P小于0.05）变化。此外，全身给予糖皮质激素对单核吞噬细胞的影响各不相同，这取决于特定的细胞位置。
