Nizhinkivs'ka T M, Ivanov O P, Korotkoruchko V P
Ukr Biokhim Zh. 1977 May-Jun;49(3):55-60.
Affinity of an acid anion for proteins has a decisive effect on conditions for detecting using an acid medium immunoglobulin G peculiar to the malignant growth in blood serum of patients with cancer. Depending on the protein affinity of the anion of the acid used for the reaction immunoglobulin G appearing in blood of patients with cancer under equal conditions may be in a soluble state or change to a sediment. The presence of salts in the reaction mixture has no effect on conditions of the reaction performance. Exposition of blood sera to the acid medium before titration favours a more distinct detection of the studied protein in blood of patients with cancer.
酸性阴离子与蛋白质的亲和力对使用酸性介质检测癌症患者血清中恶性肿瘤特有的免疫球蛋白G的条件具有决定性影响。根据用于反应的酸的阴离子与蛋白质的亲和力,在相同条件下癌症患者血液中出现的免疫球蛋白G可能处于可溶状态或转变为沉淀物。反应混合物中盐的存在对反应进行的条件没有影响。滴定前将血清暴露于酸性介质有利于更清晰地检测癌症患者血液中所研究的蛋白质。