McVay John D, Rodriguez David, Rainwater Thomas R, Dever Jennifer A, Platt Steven G, McMurry Scott T, Forstner Michael R J, Densmore Llewellyn D
Department of Biological Sciences, Texas Tech University, Lubbock, TX 79409, USA.
J Exp Zool A Ecol Genet Physiol. 2008 Dec 1;309(10):643-8. doi: 10.1002/jez.500.
Microsatellite data were generated from hatchlings collected from ten nests of Morelet's Crocodile (Crocodylus moreletii) from New River Lagoon and Gold Button Lagoon in Belize to test for evidence of multiple paternity. Nine microsatellite loci were genotyped for 188 individuals from the 10 nests, alongside 42 nonhatchlings from Gold Button Lagoon. Then mitochondrial control region sequences were generated for the nonhatchlings and for one individual from each nest to test for presence of C. acutus-like haplotypes. Analyses of five of the nine microsatellite loci revealed evidence that progeny from five of the ten nests were sired by at least two males. These data suggest the presence of multiple paternity as a mating strategy in the true crocodiles. This information may be useful in the application of conservation and management techniques to the 12 species in this genus, most of which are threatened or endangered.
从伯利兹新河泻湖和金纽扣泻湖的10个莫雷莱鳄（Crocodylus moreletii）巢穴收集的幼鳄中获取微卫星数据，以检测多重父权的证据。对来自10个巢穴的188只个体以及来自金纽扣泻湖的42只非幼鳄进行了9个微卫星位点的基因分型。然后为非幼鳄和每个巢穴的一只个体生成线粒体控制区序列，以检测是否存在似美洲鳄（C. acutus）的单倍型。对9个微卫星位点中的5个进行分析后发现，10个巢穴中有5个巢穴的后代至少由两只雄性授精。这些数据表明，多重父权作为一种交配策略存在于真鳄类中。这些信息可能有助于对该属的12个物种应用保护和管理技术，其中大多数物种受到威胁或濒危。
