基于泻下生物测定法的大黄质量评价方法学研究
[Methodological study on quality evaluation of rhubarb based on purgative bioassay].
作者信息
Li Hui-Fang, Wang Jia-Bo, Qu Yi, Jin Cheng, Wang Qiang, Xiao Xiao-He, Li Zu-Lun
机构信息
Institute of Chinese Materia Medica, 302 Hospital of PLA, Beijing 100039, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2008 Jun;33(11):1309-12.
OBJECTIVE
To establish a method suitable to determine the purgative biopotency of rhubarb and construct a new quality evaluation pattern of rhubarb.
METHOD
A series of factors such as observation index (mass of feces in 10 hours), animal strain (ICR mice), sex (male) and the dose of diphenoxylate complex (50 mg x kg(-1)) was investigated and fixed. The purgative biopotency as well as anthraquinone determination was used to evaluate the quality of different rhubarb samples.
RESULT
There wasn't a good linear relationship between the purgative biopotency and content of anthraquinone. The quality difference of rhubarb samples could be well characterized by combination of purgative biopotency determination and anthraquinone determination.
CONCLUSION
The purgative biopotency determination can be used in quality control and evaluation of rhubarb.
目的
建立一种适合测定大黄致泻生物活性的方法，并构建大黄新的质量评价模式。
方法
对一系列因素进行研究并确定，如观察指标（10小时内粪便质量）、动物品系（ICR小鼠）、性别（雄性）以及复方地芬诺酯剂量（50mg·kg⁻¹）。采用致泻生物活性及蒽醌含量测定来评价不同大黄样品的质量。
结果
大黄致泻生物活性与蒽醌含量之间不存在良好的线性关系。通过致泻生物活性测定与蒽醌含量测定相结合，能够很好地表征大黄样品的质量差异。
结论
致泻生物活性测定可用于大黄的质量控制与评价。
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