Wang Jiabo, Li Huifang, Jin Cheng, Qu Yi, Xiao Xiaohe
PLA Institute of Chinese Materia Medica, 302 Hospital of PLA, Beijing 100039, PR China.
J Pharm Biomed Anal. 2008 Aug 5;47(4-5):765-70. doi: 10.1016/j.jpba.2008.03.011. Epub 2008 Mar 20.
A reverse phase ultra performance liquid chromatography (UPLC) method was developed for the rapid quantification of five anthraquinone derivatives (aloe-emodin, rhein, emodin, chrysophanol and physcion) in rhubarb using a Waters Acquity BEH C18, 50 mm x 2.1 mm, 1.7 microm column. The runtime was as short as 3 min. The influence of flow rate and column temperature on resolution was investigated. The method was validated according to the regulatory guidelines with respect to precision, accuracy, linearity and robustness. Comparison of system performance with conventional HPLC was made with respect to analysis time, efficiency and sensitivity. The proposed method was found to be reproducible and convenient for quantitative analysis of five anthraquinone derivatives in three species of rhubarb and related preparations.
建立了一种反相超高效液相色谱（UPLC）方法，用于快速定量测定大黄中五种蒽醌衍生物（芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚），采用沃特世ACQUITY BEH C18柱（50 mm×2.1 mm，1.7μm）。运行时间短至3分钟。研究了流速和柱温对分离度的影响。该方法按照监管指南进行了精密度、准确度、线性和稳健性验证。在分析时间、效率和灵敏度方面，将该系统性能与传统HPLC进行了比较。结果表明，该方法可重现，适用于三种大黄及相关制剂中五种蒽醌衍生物的定量分析。
