通过聚合酶链反应对肾外肾素信使核糖核酸进行定量测量。

Quantitative measurement of extra-renal renin mRNA by polymerase chain reaction.

作者信息

Okura T, Kitami Y, Iwata T, Hiwada K

机构信息

2nd Department of Internal Medicine, Ehime University School of Medicine, Japan.

出版信息

Biochem Biophys Res Commun. 1991 Aug 30;179(1):25-31. doi: 10.1016/0006-291x(91)91328-a.

DOI:10.1016/0006-291x(91)91328-a

PMID:1883355

Abstract

We established a simplified method of the quantitative measurement of extra-renal renin messenger RNA using polymerase chain reaction system. We could detect the renin messenger RNA in the kidney, heart, aorta and adrenal gland from a single RNA sample obtained from a Wistar-Kyoto rat. In the kidney, heart, aorta and adrenal gland, the contents of renin messenger RNA were found to be 55.8 +/- 17.8, 0.15 +/- 0.05, 0.11 +/- 0.03 and 0.16 +/- 0.07 pg/micrograms of total RNA (n = 5, mean +/- s.d.), respectively. The present method is very useful to study the extra-renal renin-angiotensin system.

摘要

我们建立了一种使用聚合酶链反应系统定量测量肾外肾素信使核糖核酸的简化方法。我们能够从一只Wistar-Kyoto大鼠获取的单个RNA样本中检测到肾脏、心脏、主动脉和肾上腺中的肾素信使核糖核酸。在肾脏、心脏、主动脉和肾上腺中，肾素信使核糖核酸的含量分别为每微克总RNA 55.8±17.8、0.15±0.05、0.11±0.03和0.16±0.07皮克（n = 5，平均值±标准差）。本方法对于研究肾外肾素-血管紧张素系统非常有用。