肌动蛋白基因的过表达与白色念珠菌的形态发生有关。

Overexpression of the actin gene is associated with the morphogenesis of Candida albicans.

作者信息

Paranjape V, Datta A

机构信息

Molecular Biology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, India.

出版信息

Biochem Biophys Res Commun. 1991 Aug 30;179(1):423-7. doi: 10.1016/0006-291x(91)91387-r.

DOI:10.1016/0006-291x(91)91387-r

PMID:1883368

Abstract

A progressive increase in the synthesis of actin mRNA was observed by Northern blot analysis, when cells were induced to form germ tubes at 37 degrees C by N-acetyl-D-glucosamine. Presence of trifluoperazine, a calmodulin inhibitor, or incubation of cells at 25 degrees C, or by replacing N-acetyl-D-glucosamine with glucose which inhibited germ tube formation lowered this synthesis. Furthermore, in vitro translation of total RNA revealed an increase in the synthesis of actin (45 kDa) during germ tube formation. These results suggest for the first time that the expression of actin gene is regulated during morphogenesis of C. albicans.

摘要

通过Northern印迹分析观察到，当细胞在37℃被N-乙酰-D-葡萄糖胺诱导形成芽管时，肌动蛋白mRNA的合成呈渐进性增加。钙调蛋白抑制剂三氟拉嗪的存在、细胞在25℃孵育，或者用抑制芽管形成的葡萄糖替代N-乙酰-D-葡萄糖胺，均降低了这种合成。此外，总RNA的体外翻译显示在芽管形成过程中肌动蛋白（45 kDa）的合成增加。这些结果首次表明，白色念珠菌形态发生过程中肌动蛋白基因的表达受到调控。