Chaudhry P S, Newcomer P A, Casillas E R
Department of Chemistry, New Mexico State University, Las Cruces 88003.
Biochem Biophys Res Commun. 1991 Aug 30;179(1):592-8. doi: 10.1016/0006-291x(91)91412-6.
A highly purified preparation of sperm casein kinase I was obtained by sequential chromatography with phosphocellulose, gel filtration on sephacryl S-300, Affi-gel blue and DEAE-Cellulose. The chromatographic behavior and properties of the enzyme suggest that the sperm enzyme is similar to casein kinase I from other tissues. Antibodies against calf thymus casein kinase I cross-react with the sperm enzyme. A special feature of the sperm enzyme is that the activity is stimulated by spermine.
