Pitx1在发育中和再生的美西螈肢体中的表达。

Pitx1 expression in developing and regenerating axolotl limbs.

作者信息

Shimokawa Takashi, Yasutaka Satoru, Kominami Rieko, Tanaka Shigenori, Shinohara Harumichi

机构信息

Division of Anatomy II, Kanazawa Medical University, Uchinada-machi, Kahoku-gun, 920-0293 Japan.

出版信息

Okajimas Folia Anat Jpn. 2008 May;85(1):5-10. doi: 10.2535/ofaj.85.5.

DOI:10.2535/ofaj.85.5

PMID:18833906

Abstract

The expression of the homeobox transcription factor Pitx1 was investigated in the Mexican axolotl (Ambystoma mexicanum) during limb development and regeneration by whole-mount mRNA in situ hybridizations. This clone shares high amino acid identity with Pitx1 from other vertebrates (92% Xenopus; 87% chick; 75% human and mouse) within the region isolated. In the developing limbs, Pitx1 was expressed in hindlimb mesenchyme, as has been reported in other species. The expression pattern in the hindlimb might have been conserved during evolution. In the regenerating limbs, Pitx1 was expressed in both fore- and hindlimb blastemas. Our observations suggest two roles of Pitx1 in the axolotl: one is to determine the hindlimb pattern during development, and the other that relates to proliferation of regenerating tissues without regard to fore- or hindlimb.

摘要

通过全胚胎mRNA原位杂交技术，对墨西哥钝口螈（Ambystoma mexicanum）肢体发育和再生过程中同源盒转录因子Pitx1的表达进行了研究。在分离出的区域内，该克隆与其他脊椎动物的Pitx1具有较高的氨基酸同一性（与非洲爪蟾的同一性为92%；与鸡的同一性为87%；与人和小鼠的同一性为75%）。在发育中的肢体中，Pitx1在后肢间充质中表达，这与其他物种的报道一致。后肢中的表达模式可能在进化过程中得以保留。在再生肢体中，Pitx1在前肢和后肢芽基中均有表达。我们的观察结果表明，Pitx1在钝口螈中具有两种作用：一是在发育过程中确定后肢模式，二是与再生组织的增殖有关，而不考虑前肢或后肢。