Fridolf T, Ahrén B
Department of Pharmacology, Lund University, Sweden.
Biochem Biophys Res Commun. 1991 Aug 30;179(1):701-6. doi: 10.1016/0006-291x(91)91429-g.
We examined the Na(+)-dependency of the effects of GLP-1(7-36)amide in normal, overnight cultured rat islets. It was found that GLP-1(7-36)amide stimulated insulin secretion, 45Ca(2+)-efflux, and 86Rb(+)-efflux from prelabelled islets. All these effects were abolished by omitting Na+ from the medium and replacing it with N-methyl-glucamine. This suggests that GLP-1(7-36)amide stimulates insulin secretion by depolarizing the beta-cells by increasing their permeability to Na+.
我们研究了GLP-1(7-36)酰胺在正常的、过夜培养的大鼠胰岛中作用的钠离子依赖性。结果发现，GLP-1(7-36)酰胺刺激了胰岛素分泌、45Ca(2+)外流以及来自预先标记胰岛的86Rb(+)外流。通过从培养基中去除钠离子并用N-甲基葡糖胺替代它，所有这些作用都被消除了。这表明GLP-1(7-36)酰胺通过增加β细胞对钠离子的通透性使其去极化，从而刺激胰岛素分泌。
