Fontanay Stéphane, Grare Marion, Mayer Joséphine, Finance Chantal, Duval Raphaël Emmanuel
SRSMC, Nancy-University, CNRS, France.
J Ethnopharmacol. 2008 Nov 20;120(2):272-6. doi: 10.1016/j.jep.2008.09.001. Epub 2008 Sep 12.
Ursolic acid (UA), oleanolic acid (OA) and betulinic acid (BA), three hydroxyl pentacyclic triterpenoic acids (HPTAs) naturally found in a large variety of vegetarian foods, medicinal herbs and plants have been investigated for antibacterial activity.
To determine the antibacterial activity of UA, OA and BA, as well as the toxic impact on eukaryotic cells.
Minimum inhibitory concentrations were determined against five reference strains (Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 & ATCC 29213, Enterococcus faecalis ATCC 29212 and Pseudomonas aeruginosa ATCC 27853), as well as five antibiotic-resistant clinical isolates. Toxicity was evaluated against MRC-5 and HaCaT cell lines.
No antibacterial activity was observed for BA; while OA and more particularly UA, did show a moderate to good antibacterial activity, but limited to Gram-positive bacteria. Nevertheless, OA and UA were devoid of antibacterial activities against clinical isolates. Moreover, viability and cytotoxic assays demonstrated that the three compounds induced a significant cytotoxicity.
Despite of a relative similar chemical structure; UA, OA and BA harboured different antibacterial activities, with more significant ones for UA. However, considering both viability and toxicity values, these compounds seem to have a significant impact on eukaryotic cell viability.
熊果酸（UA）、齐墩果酸（OA）和桦木酸（BA）是三种羟基五环三萜酸（HPTAs），天然存在于多种素食食品、药草和植物中，已对其抗菌活性进行了研究。
确定UA、OA和BA的抗菌活性以及对真核细胞的毒性影响。
测定了对五种参考菌株（大肠杆菌ATCC 25922、金黄色葡萄球菌ATCC 25923和ATCC 29213、粪肠球菌ATCC 29212和铜绿假单胞菌ATCC 27853）以及五种耐抗生素临床分离株的最低抑菌浓度。评估了对MRC - 5和HaCaT细胞系的毒性。
未观察到BA的抗菌活性；而OA，尤其是UA，确实表现出中度至良好的抗菌活性，但仅限于革兰氏阳性菌。然而，OA和UA对临床分离株没有抗菌活性。此外，活力和细胞毒性试验表明这三种化合物均诱导了显著的细胞毒性。
尽管化学结构相对相似，但UA、OA和BA具有不同的抗菌活性，其中UA的活性更为显著。然而，综合考虑活力和毒性值，这些化合物似乎对真核细胞活力有显著影响。
