Peoples Robert C, Fried Bernard
Department of Biology, Lafayette College, Easton, PA 18042, USA.
Parasitol Res. 2009 Jan;104(2):471-3. doi: 10.1007/s00436-008-1209-8. Epub 2008 Oct 4.
This study examined the effects of long-term worm storage on the viability of Echinostoma caproni eggs. Gravid adults (referred to as worm carcasses or WCs) were maintained in Locke's 1:1 at 4 degrees C for 2, 4, 6, 8, 10, and 12 months prior to use. When used, about 100 eggs at each time interval were tested for viability by embryonating them at either 22 +/- 1 degrees C for 14 days or 28 degrees C for 10 days in artificial spring water. Viability was determined by detecting fully developed miracidia in the eggs or miracidia in the cultures at the above-mentioned time periods. There was a high percentage of fully developed eggs from WCs stored in Locke's 1:1 at 4 degrees C for 2 and 4 months, 92% and 71%, respectively. Beyond these times, there was a marked decline in egg viability. We suggest that WCs should not be stored for longer than 4 months in Locke's 1:1 at 4 degrees C to obtain optimal embryonation and hatching of E. caproni.
本研究考察了长期保存蠕虫对卡氏棘口吸虫虫卵活力的影响。将妊娠成虫（称为虫体或WCs）在4℃下以1:1的洛克氏溶液保存2、4、6、8、10和12个月后使用。使用时，将每个时间间隔的约100个虫卵置于人工泉水中，在22±1℃下孵育14天或在28℃下孵育10天，以检测其活力。通过在上述时间段内检测虫卵中发育完全的毛蚴或培养物中的毛蚴来确定活力。在4℃下以1:1的洛克氏溶液保存2个月和4个月的虫体所产的虫卵中，发育完全的虫卵比例较高，分别为92%和71%。超过这些时间后，虫卵活力显著下降。我们建议，为使卡氏棘口吸虫获得最佳的胚胎发育和孵化效果，虫体在4℃下以1:1的洛克氏溶液保存的时间不应超过4个月。
