Ma Lin, Chen Hongbing, Han Jucai, Liu Huiping
College of Agriculture, Shanxi Agricultural University, Taigu 030801, China.
Wei Sheng Wu Xue Bao. 2008 Jul;48(7):900-4.
Endophytic actinomycete Lj20 with antifungal activity was isolated from the roots of capsicum plants. We identified Lj20 and synthesized its antifungal substances.
Morphological, biological and biochemical characteristics, chemotaxonomy analysis and 16S rDNA sequences were used to identify Lj20. According to GC-MS extrapolation result, one of antifungal substances in the metabolites of Lj20 was chemically synthesized. The bioactivities were determined by mycelium growth inhibition method.
Lj20 belonged to Streptomyces sp. and was similar to Streptomyces rochei. The metabolites contained butylated hydroxytoluene and 3, 5-di-tert-butyl-4-hydroxybenzyl methyl ether. The median effective concentration (EC50) of these two compounds to Botrytis cinerea Pers. were 237.04 mg/L of the water and 186.48 mg/L of the water, respectively.
Lj20 was classified as Streptomyces rochei. Butylated hydroxytoluene and 3, 5-di-tert-butyl-4-hydroxybenzyl methyl ether had significant inhibition to the pathogen.
从辣椒植株根部分离出具有抗真菌活性的内生放线菌Lj20。我们对Lj20进行了鉴定并合成了其抗真菌物质。
利用形态学、生物学和生化特性、化学分类分析以及16S rDNA序列对Lj20进行鉴定。根据气相色谱-质谱联用（GC-MS）推断结果，化学合成了Lj20代谢产物中的一种抗真菌物质。通过菌丝生长抑制法测定生物活性。
Lj20属于链霉菌属，与罗氏链霉菌相似。代谢产物含有丁基化羟基甲苯和3,5-二叔丁基-4-羟基苄基甲基醚。这两种化合物对灰葡萄孢的半数有效浓度（EC50）分别为237.04 mg/L水和186.48 mg/L水。
Lj20被归类为罗氏链霉菌。丁基化羟基甲苯和3,5-二叔丁基-4-羟基苄基甲基醚对病原菌有显著抑制作用。
