Guo Lei, Liu He, Li Xiufen, Du Guocheng, Chen Jian
Laboratory of Environmental Biotechnology, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
Sheng Wu Gong Cheng Xue Bao. 2008 Jul;24(7):1233-9. doi: 10.1016/s1872-2075(08)60060-6.
A novel anaerobic fermentation process--multistage countercurrent fermentation was applied to improve the bioproduction of volatile fatty acids (VFAs) from excess municipal sludge. Results showed that the total VFAs concentration and the total VFAs yield reached (10.5 +/- 0.5) g/L and 0.20 gVFAs/gVS (Volatile solid) using this novel process. Comparing with the conventional anaerobic fermentation, the concentration and yield of total VFAs increased by 31% and by 54%, respectively. Moreover, removal ratio of organic solids also increased by 37% and it was 50% at the end of multistage countercurrent fermentation. We further investigated the mechanism of VFAs production. Results revealed that this novel process could reduce the inhibitory effect of VFAs on the acid-forming microorganisms, and the total VFAs yield and the removal ratio of organic solids respectively depended on the first stage and the third stage of this novel process. Therefore, the multistage countercurrent fermentation can efficiently improve the bioproduction of VFAs from excess municipal sludge, and relatively enhance the removal ratio of organic solids.
一种新型厌氧发酵工艺——多级逆流发酵被应用于提高城市剩余污泥中挥发性脂肪酸（VFAs）的生物产量。结果表明，采用这种新工艺，挥发性脂肪酸的总浓度和总产量分别达到（10.5±0.5）g/L和0.20 gVFAs/gVS（挥发性固体）。与传统厌氧发酵相比，挥发性脂肪酸的总浓度和产量分别提高了31%和54%。此外，有机固体的去除率也提高了37%，在多级逆流发酵结束时达到50%。我们进一步研究了挥发性脂肪酸产生的机制。结果表明，这种新工艺可以降低挥发性脂肪酸对产酸微生物的抑制作用，挥发性脂肪酸的总产量和有机固体的去除率分别取决于该新工艺的第一阶段和第三阶段。因此，多级逆流发酵可以有效地提高城市剩余污泥中挥发性脂肪酸的生物产量，并相对提高有机固体的去除率。
