Feng Hui-Min, He Hong-Bo, Bai Zhen, Wu Ye-Ye, Guo Bai-Dong, Zhang Ming, Zhang Xu-Dong
Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China.
Ying Yong Sheng Tai Xue Bao. 2008 Jul;19(7):1585-90.
An incubation test was conducted with mollisol applied with recommended amount of acetochlor under the conditions of sterilization, microbial inhibitor addition, and non-sterilization. During incubation, the residual amount of acetochlor and the dynamics of soil phospholipid fatty acids (PLFAs) were determined to study the relative contribution of soil microbes on the degradation of applied acetochlor, and the effects of acetochlor on the soil microbial community structure. The results showed that acetochlor was easy to be degraded by soil microbes, and bacteria contributed more than fungi. After applying acetochlor, the contents of various PLFAs changed evidently, and the soil microbial biomass indicated by C14:0, C16:0 and C18:0 was decreased. The bacterial PLFAs decreased significantly at the beginning of the incubation, but had less difference with CK (no acetochlor application) later, suggesting that bacterial activity was restored along with the degradation of acetochlor. The content of fungal PLFAs in the soil samples applied with acetochlor was lower than that of CK, implying that the inhibition of the herbicide to fungi was chronic and irreversible.
在灭菌、添加微生物抑制剂和不灭菌条件下，对添加推荐量乙草胺的软土进行了培养试验。在培养过程中，测定乙草胺残留量和土壤磷脂脂肪酸（PLFA）动态，以研究土壤微生物对施加乙草胺降解的相对贡献以及乙草胺对土壤微生物群落结构的影响。结果表明，乙草胺易于被土壤微生物降解，细菌的贡献大于真菌。施用乙草胺后，各种PLFA含量明显变化，以C14:0、C16:0和C18:0表示的土壤微生物生物量下降。培养初期细菌PLFA显著下降，但后期与对照（未施用乙草胺）差异较小，表明细菌活性随着乙草胺的降解而恢复。施用乙草胺的土壤样品中真菌PLFA含量低于对照，这意味着除草剂对真菌的抑制是长期且不可逆的。
