Wurst Martin, Robles Ana, Po June, Luu Van-Duc, Brems Stefanie, Marentije Mari, Stoitsova Savrina, Quijada Luis, Hoheisel Jörg, Stewart Mhairi, Hartmann Claudia, Clayton Christine
Zentrum für Molekulare Biologie der Universität Heidelberg (ZMBH), Im Neuenheimer Feld 282, D69120 Heidelberg, Germany.
Mol Biochem Parasitol. 2009 Jan;163(1):61-5. doi: 10.1016/j.molbiopara.2008.09.001. Epub 2008 Sep 18.
In eukaryotes, proteins containing RNA Recognition Motifs (RRMs) are involved in many different RNA processing reactions, RNA transport, and mRNA decay. Kinetoplastids rely extensively on post-transcriptional mechanisms to control gene expression, so RRM domain proteins are expected to play a prominent role. We here describe the results of an RNA interference screen targeting 37 of the 72 RRM-domain proteins of Trypanosoma brucei. RNAi targeting 8 of the genes caused clear growth inhibition in bloodstream trypanosomes, and milder effects were seen for 9 more genes. The small, single-RRM protein TbRBP3 specifically associated with 10 mRNAs in trypanosome lysates, but RBP3 depletion did not affect the transcriptome.
在真核生物中，含有RNA识别基序（RRMs）的蛋白质参与许多不同的RNA加工反应、RNA转运和mRNA降解。动质体广泛依赖转录后机制来控制基因表达，因此RRM结构域蛋白预计会发挥重要作用。我们在此描述了针对布氏锥虫72个RRM结构域蛋白中的37个进行RNA干扰筛选的结果。针对8个基因的RNA干扰在血流型锥虫中导致明显的生长抑制，另外9个基因则表现出较轻的影响。小型单RRM蛋白TbRBP3在锥虫裂解物中与10种mRNA特异性结合，但RBP3的缺失并未影响转录组。
