Arata Sayaka, Ogata Niwako, Shimozuru Michito, Takeuchi Yukari, Mori Yuji
Laboratory of Veterinary Ethology, The University of Tokyo, Tokyo, Japan.
J Vet Med Sci. 2008 Sep;70(9):971-5. doi: 10.1292/jvms.70.971.
Monoamine transporters have roles to regulate the monoamine concentrations in synaptic clefts in the central nervous system and are the targets of antidepressants and psychostimulants. They include transporters for norepinephrine, dopamine and serotonin, which are encoded by the SLC6A2, SLC6A3, and SLC6A4 genes, respectivily. We sequenced the full lengths of the coding regions of these genes for dogs and identified four single nucleotide polymorphisms (SNPs) in SLC6A2 and four in SLC6A3. One SLC6A3 SNP was non-synonymous and caused an amino acid substitution from threonine to serine. The genotype frequencies of these polymorphisms differed significantly among five breeds with different behavioral traits, suggesting that novel SLC6A2 and SLC6A3 SNPs would provide additional useful information for behavioral genetic studies in dogs.
单胺转运体在调节中枢神经系统突触间隙中的单胺浓度方面发挥作用，并且是抗抑郁药和精神兴奋剂的作用靶点。它们包括去甲肾上腺素、多巴胺和5-羟色胺的转运体，分别由SLC6A2、SLC6A3和SLC6A4基因编码。我们对犬类这些基因编码区的全长进行了测序，并在SLC6A2中鉴定出四个单核苷酸多态性（SNP），在SLC6A3中鉴定出四个。一个SLC6A3 SNP是非同义的，导致氨基酸从苏氨酸替换为丝氨酸。这些多态性的基因型频率在具有不同行为特征的五个品种之间存在显著差异，这表明新的SLC6A2和SLC6A3 SNP将为犬类行为遗传学研究提供额外的有用信息。
