p130Cas、E-钙黏蛋白和β-连环蛋白表达之间的关系、临床病理意义及在人类肝细胞癌中的预后

Relation among p130Cas, E-cadherin and beta-catenin expression, clinicopathologic significance and prognosis in human hepatocellular carcinoma.

作者信息

Guo Cheng, Liu Qing-Guang, Yang Wei, Zhang Zheng-Liang, Yao Ying-Min

机构信息

Department of Hepatobiliary Surgery, First Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China.

出版信息

Hepatobiliary Pancreat Dis Int. 2008 Oct;7(5):490-6.

PMID:18842495

Abstract

BACKGROUND

p130Cas (p130Crk-associated substance) is a junction protein that is important to the adhesion between cytoskeleton and extracellular matrix. Also, the adhesion molecules E-cadherin and beta-catenin play important roles in the invasiveness of carcinoma. This study was undertaken to investigate the effects of p130Cas, E-cadherin and beta-catenin on the invasion, metastasis and prognosis of hepatocellular carcinoma (HCC).

METHODS

Immunohistochemistry was used to evaluate the expression of p130Cas, E-cadherin, and beta-catenin in 40 patients with HCC. All patients were followed up postoperatively, and the relationship between expression and clinicopathological prognostic parameters was analyzed.

RESULTS

The positive expression rates of p130Cas and E-cadherin in HCC tissue (n=40) were 62.50% and 55.00%, but in normal liver tissue 10%, and 100%, respectively (P<0.05). The abnormal expression rate of beta-catenin in HCC tissue was 70%, while in normal liver tissue it was 13.33% (P<0.05). The positive rate of p130Cas was correlated with lymph node invasion, pathological stage, TNM stage, and a worse prognosis, but not with gender, age, HBV infection, hepatic cirrhosis, alpha-fetoprotein (AFP) level before operation, and tumor diameter. Similarly, the expression of E-cadherin and beta-catenin was correlated with lymph node invasion, pathological stage, TNM stage, and worse prognosis, but not with gender, age, HBV infection, hepatic cirrhosis, AFP level before operation, and tumor size. Correlations were found between p130Cas and abnormal E-cadherin/beta-catenin expression (P<0.001 and <0.05, respectively).

CONCLUSIONS

In HCC, there is a negative correlation between the positive expression of p130Cas and the normal expression of the adhesion molecules E-cadherin/beta-catenin, and p130Cas plays important roles in the invasion, metastasis and prognosis of HCC. p130Cas may be involved in alterating the structure and function of E-cadherin/beta-catenin, by regulating tyrosine phosphorylation via the p130Cas-Src signal pathway.

摘要

背景

p130Cas（p130Crk相关物质）是一种连接蛋白，对细胞骨架与细胞外基质之间的黏附至关重要。此外，黏附分子E-钙黏蛋白和β-连环蛋白在癌侵袭中发挥重要作用。本研究旨在探讨p130Cas、E-钙黏蛋白和β-连环蛋白对肝细胞癌（HCC）侵袭、转移及预后的影响。

方法

采用免疫组织化学法评估40例HCC患者中p130Cas、E-钙黏蛋白和β-连环蛋白的表达。所有患者术后均进行随访，并分析表达与临床病理预后参数之间的关系。

结果

HCC组织（n = 40）中p130Cas和E-钙黏蛋白的阳性表达率分别为62.50%和55.00%，而在正常肝组织中分别为10%和100%（P < 0.05）。HCC组织中β-连环蛋白的异常表达率为70%，而在正常肝组织中为13.33%（P < 0.05）。p130Cas的阳性率与淋巴结侵袭、病理分期、TNM分期及较差预后相关，但与性别、年龄、HBV感染、肝硬化、术前甲胎蛋白（AFP）水平及肿瘤直径无关。同样，E-钙黏蛋白和β-连环蛋白的表达与淋巴结侵袭、病理分期、TNM分期及较差预后相关，但与性别、年龄、HBV感染、肝硬化、术前AFP水平及肿瘤大小无关。发现p130Cas与E-钙黏蛋白/β-连环蛋白异常表达之间存在相关性（分别为P < 0.001和< 0.05）。