Department of Urology, Renmin Hospital of Wuhan University, Wuhan, Hubei, China.
Int J Urol. 2011 Sep;18(9):630-7. doi: 10.1111/j.1442-2042.2011.02793.x. Epub 2011 Jun 14.
To investigate the effects of junction protein, p130 Crk-associated substance (p130Cas), and adhesion molecules, E-cadherin and β-catenin, on the biological behavior of transitional cell carcinoma of the bladder.
In 72 paraffin embedded specimens of transitional cell carcinoma of the bladder and 20 normal controls, the expression of p130Cas, E-cadherin and β-catenin was examined by quantum dot-based immunofluorescence histochemistry (QD-IHC) and conventional immunohistochemistry (IHC).
QD-IHC was consistent with IHC in detecting the expression of the three molecules (P > 0.05 for all comparisons). The positive expression rate of p130Cas in bladder cancer tissues increased more significantly than that in normal bladder tissues (P < 0.001). Similarly, the aberrant expression rates of E-cadherin and β-catenin in bladder cancer tissues were significantly higher than those in normal bladder tissues (P < 0.001 for both comparisons). The expression of each molecule was correlated with tumor pathological grade and clinical stage (P < 0.05 for all comparisons), but not with tumor number and size (P > 0.05 for all comparisons). Furthermore, negative correlations were found between the expression intensities of p130Cas and E-cadherin or β-catenin in transitional cell carcinoma of the bladder (P < 0.05 for both comparisons).
p130Cas, E-cadherin and β-catenin might represent useful predictors of malignant degree of transitional cell carcinoma of the bladder.
研究连接蛋白 p130Crk 相关底物（p130Cas）和黏附分子 E-钙黏蛋白和β-连环蛋白对膀胱移行细胞癌生物学行为的影响。
在 72 例膀胱移行细胞癌石蜡包埋标本和 20 例正常对照中，采用量子点免疫荧光组织化学（QD-IHC）和常规免疫组织化学（IHC）检测 p130Cas、E-钙黏蛋白和β-连环蛋白的表达。
QD-IHC 与 IHC 在检测三种分子的表达方面一致（所有比较的 P 值均大于 0.05）。膀胱癌组织中 p130Cas 的阳性表达率明显高于正常膀胱组织（P<0.001）。同样，膀胱癌组织中 E-钙黏蛋白和β-连环蛋白的异常表达率明显高于正常膀胱组织（两者的 P 值均<0.001）。每种分子的表达均与肿瘤病理分级和临床分期相关（所有比较的 P 值均<0.05），但与肿瘤数量和大小无关（所有比较的 P 值均>0.05）。此外，在膀胱移行细胞癌中，p130Cas 的表达强度与 E-钙黏蛋白或β-连环蛋白的表达强度呈负相关（两者的 P 值均<0.05）。
p130Cas、E-钙黏蛋白和β-连环蛋白可能是膀胱移行细胞癌恶性程度的有用预测指标。
