Xu Luo, Zhong Feng, Guo Fei-fei, Zhao Wen-juan, Sun Xiang-rong, Wei Xiao-fang
Department of Pathology, Medical College of Qingdao University, Qingdao 266021, China.
Zhonghua Bing Li Xue Za Zhi. 2008 Apr;37(4):243-9.
To investigate the expression of motilin and its precursor mRNA in normal human thyroid. To compare the expression differences of motilin and it precursor mRNA between normal thyroid and intestines. To study the expression of motilin and its precursor mRNA in human thyroid tumors and their clinical implications.
RT-PCR, Southern blot and molecular cloning were used to detect motilin transcript expression in human thyroid and mucous membrane of small intestine. Real-time PCR and immunohistochemical techniques were used to quantify motilin precursor mRNA and motilin peptide in thyroid tissue samples including adenoma, medullary carcinoma, follicular carcinoma, papillary carcinoma and nodular goiter.
(1) The expression of motilin and its precursor mRNA in normal human thyroid was primarily in the thyroid C cells. (2) RT-PCR and Southern blot showed that motilin mRNA expressed in human thyroid was identical to that expressed in duodenum with identical sequence deposited in NCBI Genbank of America. (3) Immunohistochemistry, Western blot research and real-time PCR studies showed that motilin and its precursor mRNA were expressed in normal and tumor tissues of human thyroid. Thyroid tumors (acidophilic adenoma, medullary carcinoma, follicular carcinoma, papillary carcinoma and nodular goiter) showed intense and diffuse immunostaining for motilin peptide. Moreover, the expression of motilin and its precursor mRNA in thyroid medullar carcinoma and acidophilic adenoma were significantly higher than those of normal thyroid tissue (P < 0.05). The expression in thyroid follicular and papillary carcinomas were significantly lower than those of normal thyroid tissue (P < 0.05). There was no difference of the expression between nodular goiter and normal thyroid tissue (P > 0.05).
Motilin peptide and its precursor mRNA are expressed in C cells of human thyroid. The sequence of motilin is identical to that expressed in duodenum from NCBI Genbank of America. The expressions of both motilin and its precursor mRNA in thyroid medullary carcinoma and acidophilic adenoma are significantly increased. In contrast, their expressions in thyroid follicular and papillary carcinomas are significantly decreased. Motilin may regulate physiological functions of the thyroid through parafollicular cells. Motilin may be involved in the pathogenesis of medullary carcinoma and acidophilic adenoma of the thyroid.
研究胃动素及其前体mRNA在正常人体甲状腺中的表达。比较胃动素及其前体mRNA在正常甲状腺与肠道中的表达差异。探讨胃动素及其前体mRNA在人类甲状腺肿瘤中的表达及其临床意义。
采用逆转录聚合酶链反应（RT-PCR）、Southern印迹法和分子克隆技术检测人甲状腺及小肠黏膜中胃动素转录本的表达。运用实时荧光定量PCR和免疫组织化学技术对甲状腺组织样本（包括腺瘤、髓样癌、滤泡癌、乳头状癌和结节性甲状腺肿）中的胃动素前体mRNA和胃动素肽进行定量分析。
（1）胃动素及其前体mRNA在正常人体甲状腺中的表达主要位于甲状腺C细胞。（2）RT-PCR和Southern印迹法显示，人甲状腺中表达的胃动素mRNA与十二指肠中表达的相同，其序列已提交至美国国立生物技术信息中心（NCBI）的Genbank数据库。（3）免疫组织化学、蛋白质印迹法及实时荧光定量PCR研究表明，胃动素及其前体mRNA在人甲状腺的正常组织和肿瘤组织中均有表达。甲状腺肿瘤（嗜酸性腺瘤、髓样癌、滤泡癌、乳头状癌和结节性甲状腺肿）对胃动素肽呈强阳性且弥漫性免疫染色。此外，胃动素及其前体mRNA在甲状腺髓样癌和嗜酸性腺瘤中的表达显著高于正常甲状腺组织（P<0.05）。在甲状腺滤泡癌和乳头状癌中的表达显著低于正常甲状腺组织（P<0.05）。结节性甲状腺肿与正常甲状腺组织的表达无差异（P>0.05）。
胃动素肽及其前体mRNA在人甲状腺C细胞中表达。胃动素序列与美国NCBI Genbank数据库中十二指肠表达的序列相同。胃动素及其前体mRNA在甲状腺髓样癌和嗜酸性腺瘤中的表达显著增加。相反，它们在甲状腺滤泡癌和乳头状癌中的表达显著降低。胃动素可能通过滤泡旁细胞调节甲状腺的生理功能。胃动素可能参与甲状腺髓样癌和嗜酸性腺瘤的发病机制。
