Rodrigues Agna R S, Serrão José E, Teixeira Valéria W, Torres Jorge B, Teixeira Alvaro A
Departamento de Agronomia-Entomologia, Universidade Federal Rural de Pernambuco, Av. Dom Manoel de Medeiros, s/n, Dois Irmãos, Recife, Pernambuco 52171-900, Brazil.
J Insect Physiol. 2008 Dec;54(12):1543-51. doi: 10.1016/j.jinsphys.2008.09.003. Epub 2008 Sep 18.
Males of the predatory stinkbug, Podisus nigrispinus (Dallas) (Hemiptera: Pentatomidae), accomplish long and multiple matings. We hypothesize that this behavior is due to time requirement for spermatozoa production and their transference to the females. Thus, this work investigated the effect of mating status of males and mating duration on spermatozoa transference to the females and the location of spermatozoa in the male reproductive tract during mating. On females, morphological alterations on female spermatheca and associated structures during a mating were investigated. Analyses of male reproductive tract showed presence of spermatozoa in the lumen of vas deferens was independent of mating status (ca. virgin, 0, 12 and 24 h after having a full mating), indicating continuous spermatogenesis which is supported by the absence of a seminal vesicle for spermatozoa storage. Female spermatheca had no changes associated with the duration of mating. However, females exhibited spermathecal elastic duct swelling by 30 min of mating duration. The success of males in filling the female spermatheca with spermatozoa depends on duration of mating. Thus, the results indicate that multiple mating is a requirement for reproductive success in the species by transference of spermatozoa and accessory substances stored in the female spermathecal duct. Likewise, the long mating is a male requirement to transfer materials in appropriate amount to the female but it is not dependent on spermatozoa alone.
捕食性椿象黑角红蝽（Podisus nigrispinus (Dallas)，半翅目：蝽科）的雄性会进行长时间的多次交配。我们推测这种行为是由于精子产生及其向雌性交配转移所需的时间。因此，本研究调查了雄性交配状态和交配持续时间对精子向雌性交配转移的影响，以及交配过程中精子在雄性生殖道中的位置。在雌性方面，研究了交配过程中雌性受精囊及其相关结构的形态变化。对雄性生殖道的分析表明，输精管腔内精子的存在与交配状态无关（约为未交配、交配完全后0、12和24小时），这表明精子持续生成，且没有储存精子的精囊。雌性受精囊与交配持续时间无关。然而，交配持续30分钟时，雌性受精囊的弹性导管会肿胀。雄性用精子充满雌性受精囊的成功率取决于交配持续时间。因此，结果表明多次交配是该物种通过转移储存在雌性受精囊导管中的精子和附属物质来实现繁殖成功的必要条件。同样，长时间交配是雄性向雌性转移适量物质的必要条件，但这并不仅取决于精子。
