Shetty K V, Kedargol M R, Srinikethan G
Department of Chemical Engineering, National Institute of Technology Karnataka, Srinivasanagar Post, Surathkal, 575025, India.
Water Sci Technol. 2008;58(6):1253-9. doi: 10.2166/wst.2008.354.
Continuous aerobic biodegradation of phenol in synthetic wastewater with phenol at different concentrations (200, 300, 500, 800 and 900 ppm) was carried out in a pulsed plate column, which is used as a bioreactor with immobilised cells of Nocardia hydrocarbonoxydans (NCIM 2386) at a dilution rate of 0.4094 h(-1) and amplitude of 4.7 cm at various frequencies of pulsation (0, 0.25, 0.5, 0.75 and 1 s(-1)). The effect of frequency of pulsation on the steady state performance of the bioreactor for phenol biodegradation at different influent concentrations was studied. Percentage degradations were observed to be a combined effect of volumetric phenol loading, reactor residence time, mass transfer limitations and phenol inhibition effect. At 500 ppm influent phenol concentration the effect of frequencies of pulsation on the steady state percentage degradation at different amplitudes was studied. The percentage degradation increased with increase in frequency and almost 100% degradation was achieved at 0.75 s(-1), 0.5 s(-1) or 0.25 s(-1), with 3.3, 4.7 or 6.0 cm amplitudes respectively and hence the vibrational velocity (amplitude*frequency) was found to influence the steady state performance of the reactor. It was found that optimum vibrational velocities need to be fixed for maximum removal efficiency of the bioreactor depending on the influent phenol concentration.
在脉冲板式柱中对不同浓度（200、300、500、800和900 ppm）含苯酚的合成废水中的苯酚进行连续好氧生物降解，该脉冲板式柱用作生物反应器，固定化氧化烃诺卡氏菌（NCIM 2386）细胞，稀释率为0.4094 h⁻¹，在不同脉动频率（0、0.25、0.5、0.75和1 s⁻¹）下振幅为4.7 cm。研究了脉动频率对不同进水浓度下生物反应器苯酚生物降解稳态性能的影响。观察到降解百分比是体积苯酚负荷、反应器停留时间、传质限制和苯酚抑制作用的综合影响。在进水苯酚浓度为500 ppm时，研究了不同振幅下脉动频率对稳态降解百分比的影响。降解百分比随频率增加而增加，在0.75 s⁻¹、0.5 s⁻¹或0.25 s⁻¹时分别达到近100%降解，振幅分别为3.3、4.7或6.0 cm，因此发现振动速度（振幅×频率）会影响反应器的稳态性能。研究发现，需要根据进水苯酚浓度确定最佳振动速度，以使生物反应器的去除效率最大化。
