Department of Chemical Engineering, National Institute of Technology Karnataka Surathkal, Srinivasnagar Post, Mangalore 575025, Karnataka, India,
Bioprocess Biosyst Eng. 2011 Jan;34(1):45-56. doi: 10.1007/s00449-010-0445-3. Epub 2010 Jun 19.
A novel bioreactor called pulsed plate bioreactor (PPBR) with cell immobilised glass particles in the interplate spaces was used for continuous aerobic biodegradation of phenol present in wastewater. A mathematical model consisting of mass balance equations and accounting for simultaneous external film mass transfer, internal diffusion and reaction is presented to describe the steady-state degradation of phenol by Nocardia hydrocarbonoxydans (Nch.) in this bioreactor. The growth of Nch. on phenol was found to follow Haldane substrate inhibition model. The biokinetic parameters at a temperature of 30 ± 1 °C and pH at 7.0 ± 0.1 are μ (m) = 0.5397 h(-1), K (S) = 6.445 mg/L and K (I) = 855.7 mg/L. The mathematical model was able to predict the reactor performance, with a maximum error of 2% between the predicted and experimental percentage degradations of phenol. The biofilm internal diffusion rate was found to be the slowest step in biodegradation of phenol in a PPBR.
一种新型的生物反应器——脉冲平板生物反应器（PPBR），其在板间空间中使用固定化细胞的玻璃颗粒，用于连续好氧生物降解废水中的苯酚。本文提出了一个包含质量平衡方程的数学模型，并考虑了同时的外部膜传质、内部扩散和反应，以描述在该生物反应器中诺卡氏菌（Nocardia hydrocarbonoxydans，Nch.）对苯酚的稳态降解。研究发现，Nch. 在苯酚上的生长符合 Haldane 底物抑制模型。在 30±1°C 的温度和 7.0±0.1 的 pH 条件下，生物动力学参数为μ（m）=0.5397 h(-1)、K（S）=6.445mg/L 和 K（I）=855.7mg/L。该数学模型能够预测反应器的性能，苯酚的预测降解百分比与实验降解百分比之间的最大误差为 2%。在 PPBR 中，生物膜内部扩散速率是苯酚生物降解的最慢步骤。
