Preisinger Christian, von Kriegsheim Alex, Matallanas David, Kolch Walter
The Beatson Institute for Cancer Research, Glasgow, UK.
Proteomics. 2008 Nov;8(21):4402-15. doi: 10.1002/pmic.200800136.
Proteomics is transitioning from inventory mapping to the mapping of functional cellular contexts. This has been enabled by progress in technologies as well as conceptual strategies. Here, we review recent advances in this area with focus on cellular signalling pathways. We discuss genetics-based methods such as yeast two hybrid methods as well as biochemistry-based methods such as two-dimensional gel electrophoresis, quantitative proteomics, interaction proteomics, and phosphoproteomics. A central tenet is that by its ability to capture dynamic changes in protein expression, localisation and modification modern proteomics has become a powerful tool to map signal transduction pathways and deliver the functional information that will promote insights in cell biology and systems biology.
蛋白质组学正在从清单映射转向功能性细胞环境的映射。技术进步以及概念策略促成了这一转变。在此，我们回顾该领域的最新进展，重点关注细胞信号通路。我们讨论基于遗传学的方法，如酵母双杂交法，以及基于生物化学的方法，如二维凝胶电泳、定量蛋白质组学、相互作用蛋白质组学和磷酸化蛋白质组学。一个核心原则是，凭借其捕捉蛋白质表达、定位和修饰动态变化的能力，现代蛋白质组学已成为绘制信号转导通路并提供功能信息的强大工具，这些信息将促进对细胞生物学和系统生物学的深入理解。
