Yamazaki Chisato M, Asada Shinichi, Kitagawa Kouki, Koide Takaki
Department of Chemistry and Biochemistry, School of Advanced Science and Engineering, Waseda University, Tokyo 169-8555, Japan.
Biopolymers. 2008;90(6):816-23. doi: 10.1002/bip.21100.
Development of artificial collagens to replace the animal-derived collagens presents a challenge in the formation of safer and functional biomaterials. We report here the development of collagen-like gels by means of the self-assembly of chemically synthesized peptides. The peptides are disulfide-linked trimers of collagenous Gly-X-Y triplet repeats with self-complementary shapes. Upon cooling the peptide solutions, hydrogels of peptide supramolecules are formed by spontaneous intermolecular triple helix formation. The thermal gel-sol transition appeared to be reversible, and the transition temperatures were found to be tunable by the design of the peptides. Our systems for the formation of artificial collagen-like gels will offer possibilities for novel types of biomaterials.
开发人工胶原蛋白以替代动物源胶原蛋白，在形成更安全且具功能性的生物材料方面构成了一项挑战。我们在此报告通过化学合成肽的自组装来制备类胶原蛋白凝胶。这些肽是具有自互补形状的胶原 Gly-X-Y 三联体重复序列的二硫键连接三聚体。冷却肽溶液时，通过分子间自发形成三螺旋结构形成肽超分子水凝胶。热凝胶 - 溶胶转变似乎是可逆的，并且发现转变温度可通过肽的设计进行调节。我们用于形成人工类胶原蛋白凝胶的系统将为新型生物材料提供可能性。
