细胞黏菌盘基网柄菌细胞膜的电子自旋共振研究。

Electron spin resonance studies of the membranes of the cellular slime mold Dictyostelium discoideum.

作者信息

Von Dreele P H, Williams K L

出版信息

Biochim Biophys Acta. 1977 Jan 21;464(2):378-88. doi: 10.1016/0005-2736(77)90012-8.

DOI:10.1016/0005-2736(77)90012-8

Abstract

Doxylstearic acid spin labels are used to study the fluidity of the membranes of the cellular slime mold, Dictyostelium discoideum. The tau omicron value of the wild-type cell membrane is close to that of egg lecithin indicating a rather fluid membrane. No detectable change in the fluidity of the bulk lipids at the 16-carbon depth occurs during differentiation of the myxamoebae into stalk and spore cells despite reported changes in the individual lipid components. The results of studies on temperature-sensitive and aggregationless mutants are also presented.

摘要

多西硬脂酸自旋标记物用于研究细胞黏菌盘基网柄菌细胞膜的流动性。野生型细胞膜的τo值与卵磷脂接近，表明细胞膜相当具有流动性。尽管有报道称单个脂质成分发生了变化，但在黏变形体分化为柄细胞和孢子细胞的过程中，在16碳深度处的大量脂质的流动性没有可检测到的变化。还展示了对温度敏感和无聚集突变体的研究结果。

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