Martinson H G, Wagenaar E B
Biochim Biophys Acta. 1977 Feb 3;474(3):445-55. doi: 10.1016/0005-2787(77)90273-8.
Hydroxyapatite thermal elution chromatography was studied from an empirical standpoint. The dependence of elution temperature on elution buffer concentration was determined for various types of buffer, hydroxyapatite and nucleic acid. The results are analyzed in terms of the proper design and interpretation of thermal elution experiments. The potential for serious artifacts is demonstrated and the means by which they may be avoided is described. Various commercially available hydroxyapatites were tested in conjunction with various aqueous and partially non-aqueous buffer systems. Among the materials tested, potassium phosphate and Bio-Rad HTP were found to constitute the best buffer-hydroxyapatite system for most types of thermal elution study.
从经验角度对羟基磷灰石热洗脱色谱法进行了研究。针对各种类型的缓冲液、羟基磷灰石和核酸，测定了洗脱温度对洗脱缓冲液浓度的依赖性。根据热洗脱实验的合理设计和解释对结果进行了分析。证明了存在严重假象的可能性，并描述了避免这些假象的方法。结合各种水性和部分非水性缓冲系统对各种市售羟基磷灰石进行了测试。在测试的材料中，发现磷酸钾和伯乐HTP构成了大多数类型热洗脱研究的最佳缓冲液 - 羟基磷灰石系统。
