Nakao Ryuji, Furutsuka Kenji, Yamaguchi Masatoshi, Suzuki Kazutoshi
Molecular Imaging Center, National Institute of Radiological Sciences, Chiba 263-8555, Japan.
Nucl Med Biol. 2008 Oct;35(7):733-40. doi: 10.1016/j.nucmedbio.2008.06.005.
A sensitive quality control method is often required in positron emission tomography (PET) radiopharmaceutical analysis due to the high specific radioactivity of synthetic products. The applicability of a radio high-performance liquid chromatography (HPLC) method with fluorescence detection was evaluated for a wide variety of PET radiopharmaceuticals. In 29 different radiopharmaceuticals studied, 20 compounds exhibited native fluorescence. These properties enabled sensitive determination of their chemical masses by direct fluorimetric detection after separation by HPLC. For some substances, detection limits were below nanograms per milliliter level, at least 40 times better than current UV absorbance detection. Sufficient reproducibility and linearity were obtained for the analysis of pharmaceutical fluid. Post-column fluorimetric derivatization was also established for the quantitative determination of FDG and ClDG in [(18)F]FDG samples. These methods could be applied successfully to the analysis of PET radiopharmaceuticals with ultra-high specific radioactivity.
由于合成产品的高比放射性，在正电子发射断层扫描（PET）放射性药物分析中通常需要一种灵敏的质量控制方法。对多种PET放射性药物评估了带有荧光检测的放射性高效液相色谱（HPLC）方法的适用性。在所研究的29种不同放射性药物中，20种化合物呈现天然荧光。这些特性使得通过HPLC分离后直接荧光检测能够灵敏地测定其化学质量。对于某些物质，检测限低于每毫升纳克水平，至少比当前的紫外吸光度检测好40倍。对于药物流体分析获得了足够的重现性和线性。还建立了柱后荧光衍生化方法用于[(18)F]FDG样品中FDG和ClDG的定量测定。这些方法可成功应用于具有超高比放射性的PET放射性药物的分析。
