Nakao Ryuji, Furutuka Kenji, Yamaguchi Masatoshi, Suzuki Kazutoshi
Department of Medical Imaging, National Institute of Radiological Sciences, Chiba 263-8555, Japan.
Nucl Med Biol. 2006 Apr;33(3):441-7. doi: 10.1016/j.nucmedbio.2005.12.008. Epub 2006 Mar 9.
The usefulness of high-performance liquid chromatography with electrochemical detection (HPLC/ECD) in the quality control of positron emission tomography (PET) radiopharmaceuticals was evaluated for a number of substances. Chromatographic separation was performed using a reversed phase column and acetonitrile or 20 mM sodium phosphate buffer as the mobile phase. The effluent from the column was introduced into an electrochemical detector equipped with a glassy carbon electrode versus Ag/AgCl electrode operated in the direct current mode. In 19 of 21 PET radiopharmaceuticals studied, the compounds and corresponding precursors used in the synthesis of the radiopharmaceuticals could be successfully detected by the HPLC/ECD method. For 17 compounds with electroactive functional groups, such as aliphatic amines, phenols and aromatic amines, the detection limits were ppb levels for a 20-mul injection volume; this was significantly better compared with ultraviolet (UV) detection. This method could be applied to the analysis of [11C]MP4A, useful PET radiopharmaceutical for measuring acetylcholinesterase activity in the brain with no available UV absorbance.
对多种物质评估了高效液相色谱-电化学检测法（HPLC/ECD）在正电子发射断层扫描（PET）放射性药物质量控制中的实用性。使用反相柱，以乙腈或20 mM磷酸钠缓冲液作为流动相进行色谱分离。柱流出物被引入配备有玻碳电极对Ag/AgCl电极且以直流模式运行的电化学检测器中。在所研究的21种PET放射性药物中的19种中，通过HPLC/ECD方法能够成功检测到用于合成放射性药物的化合物及其相应前体。对于17种具有电活性官能团的化合物，如脂肪胺、酚类和芳香胺，对于20 μl进样体积，检测限为ppb水平；与紫外（UV）检测相比，这有显著改善。该方法可应用于[11C]MP4A的分析，[11C]MP4A是一种有用的PET放射性药物，可用于测量大脑中的乙酰胆碱酯酶活性，且没有可用的紫外吸收。
