Yang Wei, Guo Cheng, Liu Qing-guang, Pan Chengen
Hepatobiliary Department, First Hospital, Xi'an Jiaotong University, Xi'an, PR China.
Biomed Pharmacother. 2009 Jul;63(6):404-8. doi: 10.1016/j.biopha.2008.08.020. Epub 2008 Sep 18.
This study explores the novel H22 whole-cell vaccine of active specific immunotherapy in the treatment of hepatocellular carcinoma. H22 hepatoma tumor vaccine modified by human interleukin-2 (hIL-2) and mouse granulocyte-monocyte colony-stimulating factor (mGM-CSF) fusion gene was prepared to study its specific anti-tumor immunity. Mice were inoculated by these vaccines. Then tumor cells were injected into mouse models. The (51)Cr release assay was used to examine the cytotoxicities of the splenocytes to H22 hepatoma cells in immunized mice, tumor-bearing mice and control mice. The blood was needed to test the level of IL-10 and interferon (IFN)-gamma in serum. Survival time of mice was calculated. Specific cytotoxicity rate of splenocytes from the immunized mice to H22 cancer cell was 38%, significantly higher than 13.6% in the tumor-bearing group, 7.5% in the control group, and 9.1% in S180 cells (p<0.05). Serum IFN-gamma in the immunized group was significantly increased compared with other groups (p<0.01), and serum IL-10 in the immunized group was significantly decreased compared with other groups (p<0.01). The survival time of the transgenic vaccinated group was significantly longer.
本研究探讨新型H22全细胞疫苗主动特异性免疫疗法治疗肝细胞癌的效果。制备了经人白细胞介素-2（hIL-2）和小鼠粒细胞-巨噬细胞集落刺激因子（mGM-CSF）融合基因修饰的H22肝癌肿瘤疫苗，以研究其特异性抗肿瘤免疫。用这些疫苗接种小鼠。然后将肿瘤细胞注入小鼠模型。采用（51）Cr释放试验检测免疫小鼠、荷瘤小鼠和对照小鼠脾细胞对H22肝癌细胞的细胞毒性。采集血液检测血清中IL-10和干扰素（IFN）-γ水平。计算小鼠存活时间。免疫小鼠脾细胞对H22癌细胞的特异性细胞毒性率为38%，显著高于荷瘤组的13.6%、对照组的7.5%和S180细胞组的9.1%（p<0.05）。免疫组血清IFN-γ水平与其他组相比显著升高（p<0.01），免疫组血清IL-10水平与其他组相比显著降低（p<0.01）。转基因疫苗接种组的存活时间显著延长。
