Weiss Stéphanie, Carapito Christine, Cleiss Jessica, Koechler Sandrine, Turlin Evelyne, Coppee Jean-Yves, Heymann Michaël, Kugler Valérie, Stauffert Magalie, Cruveiller Stéphane, Médigue Claudine, Van Dorsselaer Alain, Bertin Philippe N, Arsène-Ploetze Florence
Génétique Moléculaire, Génomique et Microbiologie, Université Louis Pasteur, Strasbourg, France.
Biochimie. 2009 Feb;91(2):192-203. doi: 10.1016/j.biochi.2008.07.013. Epub 2008 Sep 25.
The arsenite-oxidizing strain Herminiimonas arsenicoxydans proteome was investigated with gel electrophoresis and tandem mass spectrometry analyses. The comparison of experimental and theoretical M(r) and pI, as well as that of peptide sequences identified by MS and predicted protein sequences, allowed the correction of five protein annotations. More importantly, the functional analysis of SDS- and 2D-PAGE proteome maps obtained in the presence of arsenic, combined with partial transcriptomic results indicate that H. arsenicoxydans expressed genes and proteins required not only for arsenic detoxification or stress response but also involved in motility, exopolysaccharide synthesis, phosphate import or energetic metabolism. This study provides therefore new insights into the adaptation processes of H. arsenicoxydans in response to arsenic.
通过凝胶电泳和串联质谱分析对砷氧化菌株砷氧化赫氏菌的蛋白质组进行了研究。对实验和理论相对分子质量（M(r)）及等电点（pI）的比较，以及对质谱鉴定的肽序列和预测的蛋白质序列的比较，修正了5个蛋白质注释。更重要的是，在有砷存在的情况下获得的十二烷基硫酸钠（SDS）和二维聚丙烯酰胺凝胶电泳（2D-PAGE）蛋白质组图谱的功能分析，结合部分转录组学结果表明，砷氧化赫氏菌表达的基因和蛋白质不仅用于砷解毒或应激反应，还参与运动性、胞外多糖合成、磷酸盐导入或能量代谢。因此，本研究为砷氧化赫氏菌响应砷的适应过程提供了新的见解。
