精胺氧化酶（SMO）活性增加作为成肌C2C12细胞的一种新型分化标志物。

Increased spermine oxidase (SMO) activity as a novel differentiation marker of myogenic C2C12 cells.

作者信息

Cervelli Manuela, Fratini Emiliano, Amendola Roberto, Bianchi Marzia, Signori Emanuela, Ferraro Elisabetta, Lisi Antonella, Federico Rodolfo, Marcocci Lucia, Mariottini Paolo

机构信息

Dipartimento di Biologia, Università Roma Tre, Rome, Italy.

出版信息

Int J Biochem Cell Biol. 2009 Apr;41(4):934-44. doi: 10.1016/j.biocel.2008.09.009. Epub 2008 Sep 21.

DOI:10.1016/j.biocel.2008.09.009

PMID:18852063

Abstract

Spermine oxidase (SMO) is a FAD-containing enzyme involved in animal cell polyamines (PA) homeostasis, selectively active on spermine and producing H(2)O(2), spermidine, and the 3-aminopropanal. In the present study, we have examined the SMO gene expression during the mouse myoblast C2C12 cell differentiation induced with two different stimuli by RT-PCR analysis, polysome-mRNP distribution and enzyme activity. SMO transcript accumulation and enzymatic activity increases during C2C12 cell differentiation and correlates with the decrease of spermine content. Many proteins are highly regulated during the phenotypic conversion of rapidly dividing C2C12 myoblasts into fully differentiated post-mitotic myotubes. The SMO gene induction represents a novel and additional marker of C2C12 cell differentiation. The sub-cellular localization of the SMOalpha and SMOmu splice variants is not involved in the differentiation processes. Nuclear localization of only the SMOmu protein was confirmed.

摘要

精胺氧化酶（SMO）是一种含黄素腺嘌呤二核苷酸（FAD）的酶，参与动物细胞多胺（PA）的稳态调节，对精胺具有选择性活性，并产生过氧化氢、亚精胺和3-氨基丙醛。在本研究中，我们通过逆转录-聚合酶链反应（RT-PCR）分析、多核糖体-信使核糖核蛋白（polysome-mRNP）分布和酶活性，研究了在两种不同刺激诱导下小鼠成肌细胞C2C12细胞分化过程中的SMO基因表达。在C2C12细胞分化过程中，SMO转录本积累和酶活性增加，且与精胺含量的降低相关。在快速分裂的C2C12成肌细胞向完全分化的有丝分裂后肌管的表型转化过程中，许多蛋白质受到高度调节。SMO基因的诱导是C2C12细胞分化的一种新的额外标志物。SMOα和SMOμ剪接变体的亚细胞定位不参与分化过程。仅证实了SMOμ蛋白的核定位。