体内大脑皮质细胞去极化过程中的脑环磷酸腺苷

Brain cyclic adenosine 3',5'-monophosphate during depolarization of the cerebral cortical cells in vivo.

作者信息

Krivánek J

出版信息

Brain Res. 1977 Jan 28;120(3):493-505. doi: 10.1016/0006-8993(77)90402-4.

Abstract

Cyclic AMP (cAMP) was determined in that part of the rat cerebral cortex which was invaded by slow potential change (SPC) accompanying cortical spreading depression (CSD). cAMP was determined at various phases of SPC development and at several time intervals after SPC recovery. At maximum SPC, cAMP was increased by 100% above control and by 116% at the time of SPC recovery. Five, 10 and 15 min after SPC recovery, +54%, +34% and 0% increase in cAMP was found, respectively. The activity of phosphorylase a increased by 112% at SPC recovery (maximal cAMP increase), and by 13% 15 min later (complete cAMP recovery). Some depolarizing agents which differ in their ability to stimulate cAMP formation in vitro (veratridine, cyanide and glutamate) when applied topically onto the cerebral cortex in concentrations evoking CSD (2 mM veratridine, 10 mM cyanide and 100 mM glutamate), induced uniform increase of cAMP by about 100%. The same cAMP augmentation was found in the cortical region under maximum SPC induced by these agents.

摘要

在大鼠大脑皮层中伴随皮层扩散性抑制（CSD）出现慢电位变化（SPC）的那部分区域测定了环磷酸腺苷（cAMP）。在SPC发展的各个阶段以及SPC恢复后的几个时间间隔测定了cAMP。在SPC达到最大值时，cAMP比对照增加了100%，在SPC恢复时增加了116%。在SPC恢复后5分钟、10分钟和15分钟，分别发现cAMP增加了54%、34%和0%。磷酸化酶a的活性在SPC恢复时（cAMP增加到最大值）增加了112%，在15分钟后（cAMP完全恢复）增加了13%。一些在体外刺激cAMP形成能力不同的去极化剂（藜芦碱、氰化物和谷氨酸），当以诱发CSD的浓度（2 mM藜芦碱、10 mM氰化物和100 mM谷氨酸）局部应用于大脑皮层时，会使cAMP均匀增加约100%。在这些药物诱导的最大SPC下的皮层区域也发现了相同的cAMP增加。