埃及血吸虫27 - 29 kDa抗原用于埃及血吸虫病免疫诊断的评估。
Evaluation of Schistosoma haematobium 27-29 kDa antigen for immunodiagnosis of schistosomiasis haematobium.
作者信息
El Amir Azza
机构信息
Department of Zoology, Faculty of Science, Cairo University, Egypt.
出版信息
J Egypt Soc Parasitol. 2008 Aug;38(2):435-51.
The study demonstrated the immunodiagnostic potential of differrent Egyptian human Schistosoma haematobium antigens. ELISA was used to measure the levels of total immunoglobulin G (IgG) and IgG4 antibodies (Abs) against S. haematobium adult worm antigens (Ags) (SAWA), excretory/secretory Ags (E/S) and cysteine proteinase Ag (27-29 kDa) for diagnosis of schistosomiasis. SDS-PAGE profiles of S. haematobium Ags showed several bands for SAWA, E/S and 27-29 kDa Ags which are characteristic of infections with Schistosoma spp. Purified protein fraction showed a single homogenous band of 27-29 kDa. For summarizing the potency of S. haematobium Ags, sensitivety rate, negative predictive value and diagnostic efficacy were calculated between data of 40 human patients in ELISA. SAWA Ag recorded 85.0 %, 77%, 90.0% with total IgG & 90.0 %, 83% and 93.3% with IgG4 isotype, respectively. While, E/S recorded 87.5%, 80%, 92.0% with total IgG and 92.5%, 87%, 95.0% with IgG4 isotype, respectively. Purified 27-29 kDa Ag presents the higher significant (P<0.01) results recording 90.0%, 83%, 93.3% with total IgG and 97.5%, 95%, 98.3% with IgG4 isotype, respectively. The results proved that combining the detection of IgG4 isotype using the 27-29 kDa Ag in sera of schistosomiasis haematobium patients in ELISA test could represent an effective immunodiagnostic tool for detecting infection in low worm burden population. This test could be useful in sero-epidemiolocal studies in low endemic areas and in diagnosis of infection in travelers to schistosomiasis endemic areas.
该研究证明了不同埃及人血吸虫抗原的免疫诊断潜力。采用酶联免疫吸附测定（ELISA）法检测针对埃及血吸虫成虫抗原（SAWA）、排泄/分泌抗原（E/S）和半胱氨酸蛋白酶抗原（27 - 29 kDa）的总免疫球蛋白G（IgG）和IgG4抗体水平，用于血吸虫病的诊断。埃及血吸虫抗原的十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS - PAGE）图谱显示，SAWA、E/S和27 - 29 kDa抗原呈现出多条条带，这些条带是血吸虫属感染的特征。纯化的蛋白组分显示出一条单一的27 - 29 kDa均匀条带。为总结埃及血吸虫抗原的效力，在ELISA检测中，对40例人类患者的数据计算敏感度、阴性预测值和诊断效能。SAWA抗原与总IgG的检测结果分别为85.0%、77%、90.0%，与IgG4同种型的检测结果分别为90.0%、83%、93.3%。而E/S与总IgG的检测结果分别为87.5%、80%、92.0%，与IgG4同种型的检测结果分别为92.5%、87%、95.0%。纯化的27 - 29 kDa抗原呈现出更高的显著（P<0.01）结果，与总IgG的检测结果分别为90.0%、83%、93.3%，与IgG4同种型的检测结果分别为97.5%、95%、98.3%。结果证明，在ELISA试验中，联合检测埃及血吸虫病患者血清中使用27 - 29 kDa抗原的IgG4同种型，可成为检测低虫负荷人群感染的有效免疫诊断工具。该试验可用于低流行地区的血清流行病学研究以及前往血吸虫病流行地区旅行者感染的诊断。
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