Salah F, El Bassiouny A, Rabia I, Demerdash Z, Roshdy M, Shaker Z
Department of Immunology, Theodor Bilharz Research Institute, Warak El Hadar, Imbaba, Giza, P.O. Box 30, Cairo, 12411, Egypt.
Parasitol Res. 2006 Oct;99(5):528-33. doi: 10.1007/s00436-005-0016-8. Epub 2006 Apr 22.
The present study was designed to prepare monoclonal antibodies (MAbs) against Schistosoma haematobium soluble egg antigen (SEA) with immunodiagnostic potential for urinary schistosomiasis. From a panel of MAbs, a pair of IgG1 MAbs (2D/11C and 10B/2C) specific for S. haematobium SEA was selected. Both MAbs recognized one band with a 42-kDa molecular weight by western blots. The pair of MAbs was employed in sandwich ELISA for the detection of circulating schistosome antigen (CSA), one as antigen-capturing antibody and the other as peroxidase-conjugated antigen-detecting antibody. The lower detection limit of the assay was 1 ng/ml of S. haematobium SEA. The assay was performed on sera of 65 S. haematobium-infected patients, 25 patients infected with other parasites (Fasciola hepatica, Echinococcus granulosus), and 20 noninfected individuals. CSA was demonstrated in 89% of the S. haematobium-infected group. However, CSA was negative in the sera of healthy individuals and patients infected with other parasites, giving an overall specificity of 100% for the CSA assay. A positive correlation (r=0.37, p<0.01) was detected between the number of S. haematobium eggs excreted in 10 ml urine and the CSA level detected in the sera of S. haematobium-infected patients. Our data show that the use of anti-S. haematobium MAbs for the detection of CSA provides a sensitive and specific method for the immunodiagnosis of active S. haematobium-infected patients. Moreover, CSA assay using this anti-S. haematobium MAb/ELISA system was proven to correlate with intensity of infection and hence morbidity assessment.
本研究旨在制备针对埃及血吸虫可溶性虫卵抗原（SEA）的单克隆抗体（MAb），用于尿路血吸虫病的免疫诊断。从一组单克隆抗体中，选择了一对对埃及血吸虫SEA具有特异性的IgG1单克隆抗体（2D/11C和10B/2C）。通过蛋白质印迹法，两种单克隆抗体均识别出一条分子量为42 kDa的条带。这对单克隆抗体用于夹心ELISA检测循环血吸虫抗原（CSA），一种作为抗原捕获抗体，另一种作为过氧化物酶偶联的抗原检测抗体。该检测方法的最低检测限为1 ng/ml埃及血吸虫SEA。对65例埃及血吸虫感染患者、25例感染其他寄生虫（肝片吸虫、细粒棘球绦虫）的患者和20例未感染个体的血清进行了检测。在89%的埃及血吸虫感染组中检测到CSA。然而，健康个体和感染其他寄生虫患者的血清中CSA呈阴性，CSA检测的总体特异性为100%。在10 ml尿液中排出的埃及血吸虫卵数量与埃及血吸虫感染患者血清中检测到的CSA水平之间检测到正相关（r = 0.37，p < 0.01）。我们的数据表明，使用抗埃及血吸虫单克隆抗体检测CSA为活动性埃及血吸虫感染患者的免疫诊断提供了一种灵敏且特异的方法。此外，使用这种抗埃及血吸虫单克隆抗体/ELISA系统进行CSA检测被证明与感染强度相关，因此与发病率评估相关。
