Huang Wenhui, Zhang Luyong, Niu Rui, Liao Hong
Neurobiology Lab, New Drug Screening Centre, China Pharmaceutical University, 1# Shen Nong road, Nanjing, 210038, People's Republic of China.
In Vitro Cell Dev Biol Anim. 2009 Jan-Feb;45(1-2):10-4. doi: 10.1007/s11626-008-9145-6. Epub 2008 Oct 15.
Extracellular matrix (ECM) molecules constitute a "niche" that modulates the migration, proliferation, and differentiation of neural stem/progenitor cells (NSPCs). The glycoprotein Tenascin-R (TN-R) is an ECM molecule, comprising multiple domains. Either the whole TN-R molecule or its distinct domains has been demonstrated to play a very important role in the developing central nervous system. However, little is known about the effect of the TN-R domain on NSPCs, especially NSPC migration. In the present study, we first show that both TN-R domains epidermal growth factor-like repeat (EGFL) and fibronectin type III (FN)6-8 can inhibit the NSPCs migration from neurospheres in vitro. Furthermore, both the EGFL and FN6-8 domains affect the distribution of neurons generated from neurospheres, indicating that EGFL and FN6-8 domains inhibit the motility of neurons generated from neurospheres. These results suggest that TN-R has an inhibitory effect on NSPCs migration.
细胞外基质（ECM）分子构成了一个“生态位”，可调节神经干/祖细胞（NSPCs）的迁移、增殖和分化。纤连蛋白-R（TN-R）是一种糖蛋白，由多个结构域组成。整个TN-R分子或其不同结构域已被证明在中枢神经系统发育中起着非常重要的作用。然而，关于TN-R结构域对NSPCs的影响，尤其是对NSPC迁移的影响，人们了解甚少。在本研究中，我们首先表明，TN-R的表皮生长因子样重复序列（EGFL）结构域和纤连蛋白III型（FN）6-8结构域均可在体外抑制神经球中NSPCs的迁移。此外，EGFL和FN6-8结构域均会影响神经球产生的神经元的分布，这表明EGFL和FN6-8结构域会抑制神经球产生的神经元的运动能力。这些结果表明，TN-R对NSPCs的迁移具有抑制作用。
